Investigations on the biosynthesis of steroids and terpenoids. XI. The 24-methylene sterol 24(28)-reductase of Saccharomyces cerevisiae

Trevor R. Jarman; A. A.Leslie Gunatilaka; David A. Widdowson

doi:10.1016/0045-2068(75)90009-7

Investigations on the biosynthesis of steroids and terpenoids. XI. The 24-methylene sterol 24(28)-reductase of Saccharomyces cerevisiae

Trevor R. Jarman
, A. A.Leslie Gunatilaka
, David A. Widdowson

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

The microsomal fraction of Saccharomyces cerevisiae has been shown to catalyse the NADPH-dependent reduction of ergosta-5,7,22,24(28)-tetraen-3β-ol to ergosterol. This cell-free system together with whole-cell cultures of polyene-resistant mutants has been used to compare the rates of reduction of other 24-methylene sterols. The results indicate that the enzyme involved exhibits a marked specificity for ergosta-5,7,22,24(28)-tetraen-3β-ol and support the concept of a major terminal step in ergosterol biosynthesis.

Original language	English (US)
Pages (from-to)	202-211
Number of pages	10
Journal	Bioorganic Chemistry
Volume	4
Issue number	2
DOIs	https://doi.org/10.1016/0045-2068(75)90009-7
State	Published - Jun 1975
Externally published	Yes

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Drug Discovery
Organic Chemistry

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10.1016/0045-2068(75)90009-7

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@article{b053629e348148f29807005450fab8ae,

title = "Investigations on the biosynthesis of steroids and terpenoids. XI. The 24-methylene sterol 24(28)-reductase of Saccharomyces cerevisiae",

abstract = "The microsomal fraction of Saccharomyces cerevisiae has been shown to catalyse the NADPH-dependent reduction of ergosta-5,7,22,24(28)-tetraen-3β-ol to ergosterol. This cell-free system together with whole-cell cultures of polyene-resistant mutants has been used to compare the rates of reduction of other 24-methylene sterols. The results indicate that the enzyme involved exhibits a marked specificity for ergosta-5,7,22,24(28)-tetraen-3β-ol and support the concept of a major terminal step in ergosterol biosynthesis.",

author = "Jarman, \{Trevor R.\} and Gunatilaka, \{A. A.Leslie\} and Widdowson, \{David A.\}",

note = "Funding Information: We thank the Nuffield Foundation and the Royal Society for financial support and Dr. U. M. Kempe, Dr. J. E. T. Corrie and Dr. B. R. Worth for preparing some of the sterols used in this study. We are grateful to Prof. Sir Derek Barton for his considerable help and encouragement throughout this work.",

year = "1975",

month = jun,

doi = "10.1016/0045-2068(75)90009-7",

language = "English (US)",

volume = "4",

pages = "202--211",

journal = "Bioorganic Chemistry",

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T1 - Investigations on the biosynthesis of steroids and terpenoids. XI. The 24-methylene sterol 24(28)-reductase of Saccharomyces cerevisiae

AU - Jarman, Trevor R.

AU - Gunatilaka, A. A.Leslie

AU - Widdowson, David A.

N1 - Funding Information: We thank the Nuffield Foundation and the Royal Society for financial support and Dr. U. M. Kempe, Dr. J. E. T. Corrie and Dr. B. R. Worth for preparing some of the sterols used in this study. We are grateful to Prof. Sir Derek Barton for his considerable help and encouragement throughout this work.

PY - 1975/6

Y1 - 1975/6

N2 - The microsomal fraction of Saccharomyces cerevisiae has been shown to catalyse the NADPH-dependent reduction of ergosta-5,7,22,24(28)-tetraen-3β-ol to ergosterol. This cell-free system together with whole-cell cultures of polyene-resistant mutants has been used to compare the rates of reduction of other 24-methylene sterols. The results indicate that the enzyme involved exhibits a marked specificity for ergosta-5,7,22,24(28)-tetraen-3β-ol and support the concept of a major terminal step in ergosterol biosynthesis.

AB - The microsomal fraction of Saccharomyces cerevisiae has been shown to catalyse the NADPH-dependent reduction of ergosta-5,7,22,24(28)-tetraen-3β-ol to ergosterol. This cell-free system together with whole-cell cultures of polyene-resistant mutants has been used to compare the rates of reduction of other 24-methylene sterols. The results indicate that the enzyme involved exhibits a marked specificity for ergosta-5,7,22,24(28)-tetraen-3β-ol and support the concept of a major terminal step in ergosterol biosynthesis.

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U2 - 10.1016/0045-2068(75)90009-7

DO - 10.1016/0045-2068(75)90009-7

M3 - Article

AN - SCOPUS:0016806399

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VL - 4

SP - 202

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JO - Bioorganic Chemistry

JF - Bioorganic Chemistry

IS - 2

ER -

Investigations on the biosynthesis of steroids and terpenoids. XI. The 24-methylene sterol 24(28)-reductase of Saccharomyces cerevisiae

Abstract

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