TY - JOUR
T1 - Investigations on the biosynthesis of steroids and terpenoids. XI. The 24-methylene sterol 24(28)-reductase of Saccharomyces cerevisiae
AU - Jarman, Trevor R.
AU - Gunatilaka, A. A.Leslie
AU - Widdowson, David A.
N1 - Funding Information:
We thank the Nuffield Foundation and the Royal Society for financial support and Dr. U. M. Kempe, Dr. J. E. T. Corrie and Dr. B. R. Worth for preparing some of the sterols used in this study. We are grateful to Prof. Sir Derek Barton for his considerable help and encouragement throughout this work.
PY - 1975/6
Y1 - 1975/6
N2 - The microsomal fraction of Saccharomyces cerevisiae has been shown to catalyse the NADPH-dependent reduction of ergosta-5,7,22,24(28)-tetraen-3β-ol to ergosterol. This cell-free system together with whole-cell cultures of polyene-resistant mutants has been used to compare the rates of reduction of other 24-methylene sterols. The results indicate that the enzyme involved exhibits a marked specificity for ergosta-5,7,22,24(28)-tetraen-3β-ol and support the concept of a major terminal step in ergosterol biosynthesis.
AB - The microsomal fraction of Saccharomyces cerevisiae has been shown to catalyse the NADPH-dependent reduction of ergosta-5,7,22,24(28)-tetraen-3β-ol to ergosterol. This cell-free system together with whole-cell cultures of polyene-resistant mutants has been used to compare the rates of reduction of other 24-methylene sterols. The results indicate that the enzyme involved exhibits a marked specificity for ergosta-5,7,22,24(28)-tetraen-3β-ol and support the concept of a major terminal step in ergosterol biosynthesis.
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U2 - 10.1016/0045-2068(75)90009-7
DO - 10.1016/0045-2068(75)90009-7
M3 - Article
AN - SCOPUS:0016806399
SN - 0045-2068
VL - 4
SP - 202
EP - 211
JO - Bioorganic Chemistry
JF - Bioorganic Chemistry
IS - 2
ER -