Investigation of lactotransferrin messenger RNA expression levels as an anti–type 2 asthma biomarker

Nicholas C. Li, Natalie Iannuzo, Stephanie A. Christenson, Paul R. Langlais, Monica Kraft, Julie G. Ledford, Xingnan Li

Research output: Contribution to journalArticlepeer-review

Abstract

Background: Lactotransferrin (LTF) has an immunomodulatory function, and its expression levels are associated with asthma susceptibility. Objectives: We sought to investigate LTF messenger RNA (mRNA) expression levels in human bronchial epithelial cells (BECs) as an anti–type 2 (T2) asthma biomarker. Methods: Association analyses between LTF mRNA expression levels in BECs and asthma-related phenotypes were performed in the Severe Asthma Research Program (SARP) cross-sectional (n = 155) and longitudinal (n = 156) cohorts using a generalized linear model. Correlation analyses of mRNA expression levels between LTF and all other genes were performed by Spearman correlation. Results: Low LTF mRNA expression levels were associated with asthma susceptibility and severity (P < .025), retrospective and prospective asthma exacerbations, and low lung function (P < 8.3 × 10−3). Low LTF mRNA expression levels were associated with high airway T2 inflammation biomarkers (sputum eosinophils and fractional exhaled nitric oxide; P < 8.3 × 10−3) but were not associated with blood eosinophils or total serum IgE. LTF mRNA expression levels were negatively correlated with expression levels of TH2 or asthma-associated genes (POSTN, NOS2, and MUC5AC) and eosinophil-related genes (IL1RL1, CCL26, and IKZF2) and positively correlated with expression levels of TH1 and inflammation genes (IL12A, MUC5B, and CC16) and TH17-driven cytokines or chemokines for neutrophils (CXCL1, CXCL6, and CSF3) (P < 3.5 × 10−6). Conclusions: Low LTF mRNA expression levels in BECs are associated with asthma susceptibility, severity, and exacerbations through upregulation of airway T2 inflammation. LTF is a potential anti-T2 biomarker, and its expression levels may help determine the balance of eosinophilic and neutrophilic asthma.

Original languageEnglish (US)
Pages (from-to)609-618
Number of pages10
JournalJournal of Allergy and Clinical Immunology
Volume154
Issue number3
DOIs
StatePublished - Sep 2024

Keywords

  • Anti-T2 biomarker
  • T2 inflammation
  • asthma exacerbations
  • asthma susceptibility
  • bronchial epithelial cells
  • eosinophils
  • lactotransferrin
  • mRNA expression
  • neutrophils
  • severe asthma

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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