Investigation of different wavelengths for scattering-based light sheet microscopy

Jingwei Zhao, Nachiket Kulkarni, Erika Dobo, Michelle J. Khan, Eric Yang, Dongkyun Kang

Research output: Contribution to journalArticlepeer-review

1 Scopus citations


Scattering-based light sheet microscopy (sLSM) is a microscopy technique that can visualize cellular morphologic details based on the scattering signal. While sLSM was previously shown to image animal tissues ex vivo at a cellular resolution, the wavelength used was chosen based on other in vivo microscopy technologies rather than through a comparison of the sLSM imaging performance between different wavelengths. In this paper, we report the development of a multi-wavelength sLSM setup that facilitates the investigation of different wavelengths for sLSM imaging. Preliminary results of imaging human anal tissues ex vivo showed that the sLSM setup allowed for comparisons of the cellular imaging performance at the same tissue location between different wavelengths. Both the quantitative analysis of the image contrast and the visual assessment by a pathologist showed that the imaging depth increased with wavelength, and the imaging depth increase was most notable around 600 nm. The preliminary results showed that the multi-wavelength sLSM setup could be useful in identifying the optimal wavelength for the specific tissue type.

Original languageEnglish (US)
Pages (from-to)3882-3892
Number of pages11
JournalBiomedical Optics Express
Issue number7
StatePublished - Jul 1 2022

ASJC Scopus subject areas

  • Biotechnology
  • Atomic and Molecular Physics, and Optics


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