TY - JOUR
T1 - Intrahost mechanisms governing emergence of resistance-breaking variants of Potato virus Y
AU - Acosta-Leal, Rodolfo
AU - Xiong, Zhongguo
N1 - Funding Information:
We are grateful to Francoise M. Blachere for her technical help and Drs. Merritt Nelson, Hans VanEtten, Judith K. Brown, and Robert McDaniel for useful discussions during the course of this work. We are also grateful to Santiago F. Elena (IBMCP-UPV Valencia, Spain) and Ivone Torres-Jerez (Noble Foundation, Inc., Ardmore, OK, USA) for critical reading of the manuscript, and Kaylee Hervey (Kansas State University) for editing and English proofreading. RAL also thanks the Mexican Scholarship Agency, CONACyT, for financial support provided during most of his Ph.D. program.
PY - 2013/3/1
Y1 - 2013/3/1
N2 - The emergence of resistance breaking (RB) variants starting from the avirulent Potato virus Y NN strain (PVYNN) was analyzed after imposing different selective host constraints. Tobacco resistance to PVYNN is conferred by va in both NC745 and VAM genotypes, but VAM carries an extra resistance gene, va2. RB-variants emerged only in NC745 and unexpectedly accumulated higher in the original host, tobacco B21, than the parental PVYNN. However, the recovery of RB-variants was interfered by PVYNN in mixed infections. Further analysis indicated that RB-variants also arose in tobacco VAM, but they were limited to subliminal local infections. Their inability to breakout was associated with absence of a mutational adaptation in the viral VPg gene, which implied a loss of fitness in tobacco B21. Altogether, the emergence of RB-variants was conditioned by inherited host constraints, interference by co-infecting avirulent virus genotypes, and fitness tradeoff of virus adaptations.
AB - The emergence of resistance breaking (RB) variants starting from the avirulent Potato virus Y NN strain (PVYNN) was analyzed after imposing different selective host constraints. Tobacco resistance to PVYNN is conferred by va in both NC745 and VAM genotypes, but VAM carries an extra resistance gene, va2. RB-variants emerged only in NC745 and unexpectedly accumulated higher in the original host, tobacco B21, than the parental PVYNN. However, the recovery of RB-variants was interfered by PVYNN in mixed infections. Further analysis indicated that RB-variants also arose in tobacco VAM, but they were limited to subliminal local infections. Their inability to breakout was associated with absence of a mutational adaptation in the viral VPg gene, which implied a loss of fitness in tobacco B21. Altogether, the emergence of RB-variants was conditioned by inherited host constraints, interference by co-infecting avirulent virus genotypes, and fitness tradeoff of virus adaptations.
KW - Cell-to-cell movement
KW - Clonal interference
KW - EIF4E
KW - Hidden virulence
KW - Potyvirus
KW - Quasispecies
KW - Random drift
KW - Recessive resistance
KW - Replicative fidelity
KW - Selection
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U2 - 10.1016/j.virol.2012.12.001
DO - 10.1016/j.virol.2012.12.001
M3 - Article
C2 - 23332684
AN - SCOPUS:84873077606
SN - 0042-6822
VL - 437
SP - 39
EP - 47
JO - Virology
JF - Virology
IS - 1
ER -