Intracellular energy failure does not underlie hyperthermic spreading depressions in immature rat hippocampal slice

Jie Wu; Teruko Takeo; Makoto Wakui; Kevin Ellsworth; Robert S. Fisher

doi:10.1016/S0006-8993(03)03355-9

Intracellular energy failure does not underlie hyperthermic spreading depressions in immature rat hippocampal slice

Jie Wu
, Teruko Takeo
, Makoto Wakui
, Kevin Ellsworth
, Robert S. Fisher

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Hyperthermic spreading depression (HSD) in immature rat hippocampal slices is mediated by Na⁺/K⁺-ATPase failure. Here, we test whether depleting intracellular ATP serves as a possible mechanism for HSD genesis. Results indicate that (1) pre-incubation with 3 mM creatine for 3 h failed to prevent hyperthermic spreading depression occurrence; and (2) intracellular ATP concentration doubled during experimental hyperthermia. This study suggests that HSD is not be mediated by depletion of intracellular ATP during hyperthermia.

Original language	English (US)
Pages (from-to)	240-243
Number of pages	4
Journal	Brain Research
Volume	987
Issue number	2
DOIs	https://doi.org/10.1016/S0006-8993(03)03355-9
State	Published - Oct 17 2003
Externally published	Yes

Keywords

Creatine
Hippocampal slice
Hyperthermia
Intracellular ATP
Na/K-ATPase
Spreading depression

ASJC Scopus subject areas

General Neuroscience
Molecular Biology
Clinical Neurology
Developmental Biology

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10.1016/S0006-8993(03)03355-9

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title = "Intracellular energy failure does not underlie hyperthermic spreading depressions in immature rat hippocampal slice",

abstract = "Hyperthermic spreading depression (HSD) in immature rat hippocampal slices is mediated by Na+/K+-ATPase failure. Here, we test whether depleting intracellular ATP serves as a possible mechanism for HSD genesis. Results indicate that (1) pre-incubation with 3 mM creatine for 3 h failed to prevent hyperthermic spreading depression occurrence; and (2) intracellular ATP concentration doubled during experimental hyperthermia. This study suggests that HSD is not be mediated by depletion of intracellular ATP during hyperthermia.",

keywords = "Creatine, Hippocampal slice, Hyperthermia, Intracellular ATP, Na/K-ATPase, Spreading depression",

author = "Jie Wu and Teruko Takeo and Makoto Wakui and Kevin Ellsworth and Fisher, \{Robert S.\}",

note = "Funding Information: This work was supported by the Marjorie Newsome and Sandra Solheim Aiken Funds for Epilepsy and the Women{\textquoteright}s Board of the Barrow Neurological Institute. J.W. was supported by the Seed Foundation of Bioengineering, Arizona State University. R.S.F. was supported by the Maslah Saul, M.D., Chair and the James and Carrie Anderson Laboratory fund for epilepsy research. K.E. was supported in part by a grant from the Howard Hughes Medical Institute.",

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doi = "10.1016/S0006-8993(03)03355-9",

language = "English (US)",

volume = "987",

pages = "240--243",

journal = "Brain Research",

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TY - JOUR

T1 - Intracellular energy failure does not underlie hyperthermic spreading depressions in immature rat hippocampal slice

AU - Wu, Jie

AU - Takeo, Teruko

AU - Wakui, Makoto

AU - Ellsworth, Kevin

AU - Fisher, Robert S.

N1 - Funding Information: This work was supported by the Marjorie Newsome and Sandra Solheim Aiken Funds for Epilepsy and the Women’s Board of the Barrow Neurological Institute. J.W. was supported by the Seed Foundation of Bioengineering, Arizona State University. R.S.F. was supported by the Maslah Saul, M.D., Chair and the James and Carrie Anderson Laboratory fund for epilepsy research. K.E. was supported in part by a grant from the Howard Hughes Medical Institute.

PY - 2003/10/17

Y1 - 2003/10/17

N2 - Hyperthermic spreading depression (HSD) in immature rat hippocampal slices is mediated by Na+/K+-ATPase failure. Here, we test whether depleting intracellular ATP serves as a possible mechanism for HSD genesis. Results indicate that (1) pre-incubation with 3 mM creatine for 3 h failed to prevent hyperthermic spreading depression occurrence; and (2) intracellular ATP concentration doubled during experimental hyperthermia. This study suggests that HSD is not be mediated by depletion of intracellular ATP during hyperthermia.

AB - Hyperthermic spreading depression (HSD) in immature rat hippocampal slices is mediated by Na+/K+-ATPase failure. Here, we test whether depleting intracellular ATP serves as a possible mechanism for HSD genesis. Results indicate that (1) pre-incubation with 3 mM creatine for 3 h failed to prevent hyperthermic spreading depression occurrence; and (2) intracellular ATP concentration doubled during experimental hyperthermia. This study suggests that HSD is not be mediated by depletion of intracellular ATP during hyperthermia.

KW - Creatine

KW - Hippocampal slice

KW - Hyperthermia

KW - Intracellular ATP

KW - Na/K-ATPase

KW - Spreading depression

UR - https://www.scopus.com/pages/publications/0142038896

UR - https://www.scopus.com/pages/publications/0142038896#tab=citedBy

U2 - 10.1016/S0006-8993(03)03355-9

DO - 10.1016/S0006-8993(03)03355-9

M3 - Article

C2 - 14499969

AN - SCOPUS:0142038896

SN - 0006-8993

VL - 987

SP - 240

EP - 243

JO - Brain Research

JF - Brain Research

IS - 2

ER -

Intracellular energy failure does not underlie hyperthermic spreading depressions in immature rat hippocampal slice

Abstract

Keywords

ASJC Scopus subject areas

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