Intracellular compartmentation of glutathione in rabbit renal proximal tubules

Rick G. Schnellmann; Lazaro J. Mandel

doi:10.1016/0006-291X(85)91235-5

Intracellular compartmentation of glutathione in rabbit renal proximal tubules

Rick G. Schnellmann
, Lazaro J. Mandel

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

The intracellular compartmentation of glutathione in rabbit renal proximal tubules was determined using digitonin, the non-ionic detergent Lubrol PX, and rapid centrifugation. Glutathione was distributed between two pools within the proximal tubules. The mitochondrial pool was the largest, containing 72 percent of the cellular glutathione while the cytoplasmic pool contained the remaining 28 percent. These results are in marked contrast to rat hepatocytes in which 85 percent of the cellular glutathione is cytoplasmic and only 15 percent is mitochondrial (1).

Original language	English (US)
Pages (from-to)	1001-1005
Number of pages	5
Journal	Biochemical and Biophysical Research Communications
Volume	133
Issue number	3
DOIs	https://doi.org/10.1016/0006-291X(85)91235-5
State	Published - Dec 31 1985
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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@article{579f187da7a348fcb719ef68574bf14e,

title = "Intracellular compartmentation of glutathione in rabbit renal proximal tubules",

abstract = "The intracellular compartmentation of glutathione in rabbit renal proximal tubules was determined using digitonin, the non-ionic detergent Lubrol PX, and rapid centrifugation. Glutathione was distributed between two pools within the proximal tubules. The mitochondrial pool was the largest, containing 72 percent of the cellular glutathione while the cytoplasmic pool contained the remaining 28 percent. These results are in marked contrast to rat hepatocytes in which 85 percent of the cellular glutathione is cytoplasmic and only 15 percent is mitochondrial (1).",

author = "Schnellmann, \{Rick G.\} and Mandel, \{Lazaro J.\}",

note = "Funding Information: Susan The Murdaugh authors for would their like excellent to thank technical Fontaine assistance Ewe11 and and Elizabeth Holmes for processing the manuscript. Supported by NIH grants ES-05329 and AM-28616 and a grant from the American Heart Association, North Carolina Affiliate.",

year = "1985",

month = dec,

day = "31",

doi = "10.1016/0006-291X(85)91235-5",

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volume = "133",

pages = "1001--1005",

journal = "Biochemical and Biophysical Research Communications",

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T1 - Intracellular compartmentation of glutathione in rabbit renal proximal tubules

AU - Schnellmann, Rick G.

AU - Mandel, Lazaro J.

N1 - Funding Information: Susan The Murdaugh authors for would their like excellent to thank technical Fontaine assistance Ewe11 and and Elizabeth Holmes for processing the manuscript. Supported by NIH grants ES-05329 and AM-28616 and a grant from the American Heart Association, North Carolina Affiliate.

PY - 1985/12/31

Y1 - 1985/12/31

N2 - The intracellular compartmentation of glutathione in rabbit renal proximal tubules was determined using digitonin, the non-ionic detergent Lubrol PX, and rapid centrifugation. Glutathione was distributed between two pools within the proximal tubules. The mitochondrial pool was the largest, containing 72 percent of the cellular glutathione while the cytoplasmic pool contained the remaining 28 percent. These results are in marked contrast to rat hepatocytes in which 85 percent of the cellular glutathione is cytoplasmic and only 15 percent is mitochondrial (1).

AB - The intracellular compartmentation of glutathione in rabbit renal proximal tubules was determined using digitonin, the non-ionic detergent Lubrol PX, and rapid centrifugation. Glutathione was distributed between two pools within the proximal tubules. The mitochondrial pool was the largest, containing 72 percent of the cellular glutathione while the cytoplasmic pool contained the remaining 28 percent. These results are in marked contrast to rat hepatocytes in which 85 percent of the cellular glutathione is cytoplasmic and only 15 percent is mitochondrial (1).

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UR - https://www.scopus.com/pages/publications/0022321345#tab=citedBy

U2 - 10.1016/0006-291X(85)91235-5

DO - 10.1016/0006-291X(85)91235-5

M3 - Article

C2 - 4084299

AN - SCOPUS:0022321345

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VL - 133

SP - 1001

EP - 1005

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 3

ER -

Intracellular compartmentation of glutathione in rabbit renal proximal tubules

Abstract

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