The effect of intracellular calcium chelators on rabbit renal proximal tubule (RPT) cell death induced by t‐butyl hydroperoxide (TBHP) and H2O2 was examined. Preincubation of RPT suspensions with 50 μM QUIN 2/AM completely prevented TBHP (0.5 mM) and H2O2 (2 mM) induced cell death [i.e., release of lactate dehydrogenase (LDH)]. QUIN 2/AM, BAPTA/AM, EGTA/AM, and FURA 2/AM, at 5 μM, decreased LDH release (at 6 hr) from 41% to 4%, 21%, 26%, and 33%, and decreased lipid peroxidation (at 1 hr) from 1.0 to 0.1, 0.4, 0.6, and 0.8 nmol MDA/mg protein, respectively, after TBHP exposure. Since oxidant‐induced lipid peroxidation and cell death are iron‐dependent in this model, these results suggest that the intracellular calcium chelators inhibit cell death by chelating iron.
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