TY - JOUR
T1 - Intestinal parasites in patients with diarrhea and human immunodeficiency virus infection in Zimbabwe
AU - Gumbo, Tawanda
AU - Sarbah, Steedman
AU - Gangaidzo, Innocent T.
AU - Ortega, Ynes
AU - Sterling, Charles R.
AU - Carville, Angela
AU - Tzipori, Saul
AU - Wiest, Peter M.
PY - 1999
Y1 - 1999
N2 - Objectives: To determine the prevalence of intestinal parasites and risk factors for infection associated with diarrhea in HIV-infected patients in Harare, Zimbabwe. Design: Prospective observational study. Methods: Single stool samples were collected from 88 HIV-infected individuals presenting with diarrhea of greater than 1 week duration. Stools were examined for intestinal parasites using modified acid last stain, fluorescence-labeled monoclonal antibody for Cryptosporidium parvum, as well as a modified trichrome stain and a PCR-based protocol for Enterocytozoon bieneusi. Results: C. parvum was detected in 9% (seven out of 82) of samples evaluated, but no Cyclospora was detected. E. bieneusi was detected in 18% (10 out of 55) of stool by trichrome staining and in 51% (28 out of 55) of stool examined by PCR. Risk factors for E. bieneusi infection were: living in rural areas, consumption of nonpiped water, contact with cow dung and household contact with an individual with diarrhea. Conclusion: E. bieneusi infection was common in HIV-infected patients with diarrhea in Zimbabwe and may be acquired through person-to-person and fecal-oral transmission.
AB - Objectives: To determine the prevalence of intestinal parasites and risk factors for infection associated with diarrhea in HIV-infected patients in Harare, Zimbabwe. Design: Prospective observational study. Methods: Single stool samples were collected from 88 HIV-infected individuals presenting with diarrhea of greater than 1 week duration. Stools were examined for intestinal parasites using modified acid last stain, fluorescence-labeled monoclonal antibody for Cryptosporidium parvum, as well as a modified trichrome stain and a PCR-based protocol for Enterocytozoon bieneusi. Results: C. parvum was detected in 9% (seven out of 82) of samples evaluated, but no Cyclospora was detected. E. bieneusi was detected in 18% (10 out of 55) of stool by trichrome staining and in 51% (28 out of 55) of stool examined by PCR. Risk factors for E. bieneusi infection were: living in rural areas, consumption of nonpiped water, contact with cow dung and household contact with an individual with diarrhea. Conclusion: E. bieneusi infection was common in HIV-infected patients with diarrhea in Zimbabwe and may be acquired through person-to-person and fecal-oral transmission.
KW - Cryptosporidium parvum
KW - Cyclospora
KW - Diarrhea
KW - Enterocytozoon bieneusi
KW - HIV
KW - Zimbabwe
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U2 - 10.1097/00002030-199905070-00011
DO - 10.1097/00002030-199905070-00011
M3 - Article
C2 - 10357381
AN - SCOPUS:0032950681
SN - 0269-9370
VL - 13
SP - 819
EP - 821
JO - AIDS
JF - AIDS
IS - 7
ER -