Interspecies Variation Affects Islet Amyloid Polypeptide Membrane Binding

Henry M. Sanders, Farzaneh Chalyavi, Caitlyn R. Fields, Marius M. Kostelic, Ming Hao Li, Daniel P. Raleigh, Martin T. Zanni, Michael T. Marty

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

The aggregation of islet amyloid polypeptide (IAPP) is associated with β-cell dysfunction in type 2 diabetes (T2D) in humans. One possible mechanism of toxicity is the interaction of IAPP oligomers with lipid membranes to disrupt the bilayer integrity and/or homeostasis of the cell. Amino acid sequence variations of IAPPs between species can greatly decrease their propensity for aggregation. For example, human IAPP is toxic to β-cells, but rat and pig IAPP are not. However, it is not clear how these differences affect membrane association. Using native mass spectrometry with lipid nanodiscs, we explored the differences in the association of human, rat, and pig IAPP with lipid bilayers. We discovered that human and rat IAPP bound nanodiscs with anionic dipalmitoyl-phosphatidylglycerol (DPPG) lipids, but pig IAPP did not. Furthermore, human and rat IAPP interacted differently with the membrane. Human IAPP show potential tetramer complexes, but rat IAPP associated with the membrane sequentially. Thus, overall IAPP-bilayer interactions are not necessarily related to disease, but small differences in oligomeric behavior at the membrane may instead play a role.

Original languageEnglish (US)
Pages (from-to)986-990
Number of pages5
JournalJournal of the American Society for Mass Spectrometry
Volume34
Issue number6
DOIs
StatePublished - Jun 7 2023
Externally publishedYes

ASJC Scopus subject areas

  • Structural Biology
  • Spectroscopy

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