Interpretation and deconvolution of nanodisc native mass spectra

Michael T. Marty, Hao Zhang, Weidong Cui, Michael L. Gross, Stephen G. Sligar

Research output: Contribution to journalArticlepeer-review

39 Scopus citations

Abstract

Nanodiscs are a promising system for studying gas-phase and solution complexes of membrane proteins and lipids. We previously demonstrated that native electrospray ionization allows mass spectral analysis of intact Nanodisc complexes at single lipid resolution. This report details an improved theoretical framework for interpreting and deconvoluting native mass spectra of Nanodisc lipoprotein complexes. In addition to the intrinsic lipid count and charge distributions, Nanodisc mass spectra are significantly shaped by constructive overlap of adjacent charge states at integer multiples of the lipid mass. We describe the mathematical basis for this effect and develop a probability-based algorithm to deconvolute the underlying mass and charge distributions. The probability-based deconvolution algorithm is applied to a series of dimyristoylphosphatidylcholine Nanodisc native mass spectra and used to provide a quantitative picture of the lipid loss in gas-phase fragmentation. [Figure not available: see fulltext.]

Original languageEnglish (US)
Pages (from-to)269-277
Number of pages9
JournalJournal of the American Society for Mass Spectrometry
Volume25
Issue number2
DOIs
StatePublished - Feb 2014
Externally publishedYes

Keywords

  • Mass spectrometry
  • Membrane proteins
  • Nanodisc

ASJC Scopus subject areas

  • Structural Biology
  • Spectroscopy

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