Interlaboratory Comparison of Branched GDGT Temperature and pH Proxies Using Soils and Lipid Extracts

Cindy De Jonge, Francien Peterse, Klaas G.J. Nierop, Thomas M. Blattmann, Marcelo Alexandre, Salome Ansanay-Alex, Thomas Austin, Mathieu Babin, Edouard Bard, Thorsten Bauersachs, Jerome Blewett, Brenna Boehman, Isla S. Castañeda, Junhui Chen, Martina L.G. Conti, Sergio Contreras, Julia Cordes, Nina Davtian, Bart van Dongen, Bella DuncanFelix J. Elling, Valier Galy, Shaopeng Gao, Jens Hefter, Kai Uwe Hinrichs, Mitchell R. Helling, Mariska Hoorweg, Ellen Hopmans, Juzhi Hou, Yongsong Huang, Arnaud Huguet, Guodong Jia, Cornelia Karger, Brendan J. Keely, Stephanie Kusch, Hui Li, Jie Liang, Julius S. Lipp, Weiguo Liu, Hongxuan Lu, Kai Mangelsdorf, Hayley Manners, Alfredo Martinez Garcia, Guillemette Menot, Gesine Mollenhauer, B. David A. Naafs, Sebastian Naeher, Lauren K. O'Connor, Ethan M. Pearce, Ann Pearson, Zhiguo Rao, Marta Rodrigo-Gámiz, Chris Rosendahl, Frauke Rostek, Rui Bao, Prasanta Sanyal, Florence Schubotz, Wesley Scott, Rahul Sen, Appy Sluijs, Rienk Smittenberg, Ioana Stefanescu, Jia Sun, Paul Sutton, Jess Tierney, Eduardo Tejos, Joan Villanueva, Huanye Wang, Josef Werne, Masanobu Yamamoto, Huan Yang, Aifeng Zhou

Research output: Contribution to journalArticlepeer-review

Abstract

Ratios of glycerol dialkyl glycerol tetraethers (GDGT), which are membrane lipids of bacteria and archaea, are at the base of several paleoenvironmental proxies. They are frequently applied to soils as well as lake- and marine sediments to generate records of past temperature and soil pH. To derive meaningful environmental information from these reconstructions, high analytical reproducibility is required. Based on submitted results by 39 laboratories from across the world, which employ a diverse range of analytical and quantification methods, we explored the reproducibility of brGDGT-based proxies (MBT′5ME, IR, and #ringstetra) measured on four soil samples and four soil lipid extracts. Correct identification and integration of 5- and 6-methyl brGDGTs is a prerequisite for the robust calculation of proxy values, but this can be challenging as indicated by the large inter-interlaboratory variation. The exclusion of statistical outliers improves the reproducibility, where the remaining uncertainty translates into a temperature offset from median proxy values of 0.3–0.9°C and a pH offset of 0.05–0.3. There is no apparent systematic impact of the extraction method and sample preparation steps on the brGDGT ratios. Although reported GDGT concentrations are generally consistent within laboratories, they vary greatly between laboratories. This large variability in brGDGT quantification may relate to variations in ionization efficiency or specific mass spectrometer settings possibly impacting the response of brGDGTs masses relative to that of the internal standard used. While ratio values of GDGT are generally comparable, quantities can currently not be compared between laboratories.

Original languageEnglish (US)
Article numbere2024GC011583
JournalGeochemistry, Geophysics, Geosystems
Volume25
Issue number7
DOIs
StatePublished - Jul 2024
Externally publishedYes

Keywords

  • GDGT
  • interlaboratory comparison
  • round robin

ASJC Scopus subject areas

  • Geophysics
  • Geochemistry and Petrology

Fingerprint

Dive into the research topics of 'Interlaboratory Comparison of Branched GDGT Temperature and pH Proxies Using Soils and Lipid Extracts'. Together they form a unique fingerprint.

Cite this