Interferon γ induces the expression of p21(waf-1) and arrests macrophage cell cycle, preventing induction of apoptosis

Jordi Xaus, Marina Cardó, Annabel F. Valledor, Concepció Soler, Jorge Lloberas, Antonio Celada

Research output: Contribution to journalArticlepeer-review

158 Scopus citations

Abstract

Incubation of bone marrow macrophages with lipopolysaccharide (LPS) or interferon γ(IFNγ) blocks macrophage proliferation. LPS treatment or M-CSF withdrawal arrests the cell cycle at early G1 and induces apoptosis. Treatment of macrophages with IFNγ stops the cell cycle later, at the G1/S boundary, induces p21(Waf1), and does not induce apoptosis. Moreover, pretreatment of macrophages with IFNγ protects from apoptosis induced by several stimuli. Inhibition of p21(Waf1) with antisense oligonucleotides or using KO mice shows that the induction of p21(Waf1) by IFNγ mediates this protection. Thus, IFNγ makes macrophages unresponsive to apoptotic stimuli by inducing p21(Waf1) and arresting the cell cycle at the G1/S boundary. Therefore, the cells of the innate immune system could only survive while they were functionally active.

Original languageEnglish (US)
Pages (from-to)103-113
Number of pages11
JournalImmunity
Volume11
Issue number1
DOIs
StatePublished - Jul 1999
Externally publishedYes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Infectious Diseases

Fingerprint

Dive into the research topics of 'Interferon γ induces the expression of p21(waf-1) and arrests macrophage cell cycle, preventing induction of apoptosis'. Together they form a unique fingerprint.

Cite this