Interaction of ovine somatomedin and multiplication stimulating activity/rat insulin-like growth factor II with cultured skeletal muscle satellite cells

M. V. Dodson; R. E. Allen; N. Shimizu; Y. Shimizu; K. L. Hossner

doi:10.1530/acta.0.1160425

Interaction of ovine somatomedin and multiplication stimulating activity/rat insulin-like growth factor II with cultured skeletal muscle satellite cells

M. V. Dodson, R. E. Allen, N. Shimizu, Y. Shimizu, K. L. Hossner

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

The interactions of ¹²⁵I-multiplication stimulating activity (MSA) and ¹²⁵I-ovine somatomedin with receptors on skeletal muscle satellite cells are described. Specific binding of ¹²⁵I-MSA/rIGF-II was inhibited by MSA/rIGF-II and oSm but not by insulin. Binding of ¹²⁵I-oSm was inhibited by MSA/rIGF-II, oSm and insulin. In addition, 24-h pre-incubation of satellite cells with insulin increased the amount of ¹²⁵I-MSA/rIGF-II bound, but insulin concentrations below 550 μg/l had no effect on the subsequent binding of ¹²⁵I-oSm. Pre-incubation of cultures with oSm or MSA/rIGF-II decreased the subsequent binding of ¹²⁵I-oSm and ¹²⁵I-MSA/rIGF-II. These preliminary experiments suggest that oSm is similar to IGF-I in its binding characteristics and that primary cultures of skeletal muscle satellite cells possess type I and type II IGF receptors.

Original language	English (US)
Pages (from-to)	425-432
Number of pages	8
Journal	Acta Endocrinologica
Volume	116
Issue number	3
DOIs	https://doi.org/10.1530/acta.0.1160425
State	Published - 1987
Externally published	Yes

ASJC Scopus subject areas

Endocrinology

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title = "Interaction of ovine somatomedin and multiplication stimulating activity/rat insulin-like growth factor II with cultured skeletal muscle satellite cells",

abstract = "The interactions of 125I-multiplication stimulating activity (MSA) and 125I-ovine somatomedin with receptors on skeletal muscle satellite cells are described. Specific binding of 125I-MSA/rIGF-II was inhibited by MSA/rIGF-II and oSm but not by insulin. Binding of 125I-oSm was inhibited by MSA/rIGF-II, oSm and insulin. In addition, 24-h pre-incubation of satellite cells with insulin increased the amount of 125I-MSA/rIGF-II bound, but insulin concentrations below 550 μg/l had no effect on the subsequent binding of 125I-oSm. Pre-incubation of cultures with oSm or MSA/rIGF-II decreased the subsequent binding of 125I-oSm and 125I-MSA/rIGF-II. These preliminary experiments suggest that oSm is similar to IGF-I in its binding characteristics and that primary cultures of skeletal muscle satellite cells possess type I and type II IGF receptors.",

author = "Dodson, {M. V.} and Allen, {R. E.} and N. Shimizu and Y. Shimizu and Hossner, {K. L.}",

year = "1987",

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T1 - Interaction of ovine somatomedin and multiplication stimulating activity/rat insulin-like growth factor II with cultured skeletal muscle satellite cells

AU - Dodson, M. V.

AU - Allen, R. E.

AU - Shimizu, N.

AU - Shimizu, Y.

AU - Hossner, K. L.

PY - 1987

Y1 - 1987

N2 - The interactions of 125I-multiplication stimulating activity (MSA) and 125I-ovine somatomedin with receptors on skeletal muscle satellite cells are described. Specific binding of 125I-MSA/rIGF-II was inhibited by MSA/rIGF-II and oSm but not by insulin. Binding of 125I-oSm was inhibited by MSA/rIGF-II, oSm and insulin. In addition, 24-h pre-incubation of satellite cells with insulin increased the amount of 125I-MSA/rIGF-II bound, but insulin concentrations below 550 μg/l had no effect on the subsequent binding of 125I-oSm. Pre-incubation of cultures with oSm or MSA/rIGF-II decreased the subsequent binding of 125I-oSm and 125I-MSA/rIGF-II. These preliminary experiments suggest that oSm is similar to IGF-I in its binding characteristics and that primary cultures of skeletal muscle satellite cells possess type I and type II IGF receptors.

AB - The interactions of 125I-multiplication stimulating activity (MSA) and 125I-ovine somatomedin with receptors on skeletal muscle satellite cells are described. Specific binding of 125I-MSA/rIGF-II was inhibited by MSA/rIGF-II and oSm but not by insulin. Binding of 125I-oSm was inhibited by MSA/rIGF-II, oSm and insulin. In addition, 24-h pre-incubation of satellite cells with insulin increased the amount of 125I-MSA/rIGF-II bound, but insulin concentrations below 550 μg/l had no effect on the subsequent binding of 125I-oSm. Pre-incubation of cultures with oSm or MSA/rIGF-II decreased the subsequent binding of 125I-oSm and 125I-MSA/rIGF-II. These preliminary experiments suggest that oSm is similar to IGF-I in its binding characteristics and that primary cultures of skeletal muscle satellite cells possess type I and type II IGF receptors.

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Interaction of ovine somatomedin and multiplication stimulating activity/rat insulin-like growth factor II with cultured skeletal muscle satellite cells

Abstract

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