Interaction of multiplication stimulating activity/rat insulin-like growth factor II with skeletal muscle satellite cells during aging

Michael V. Dodson; Ronald E. Allen

doi:10.1016/0047-6374(87)90003-0

Interaction of multiplication stimulating activity/rat insulin-like growth factor II with skeletal muscle satellite cells during aging

Michael V. Dodson, Ronald E. Allen

Research output: Contribution to journal › Article › peer-review

39 Scopus citations

Abstract

Satellite cells were isolated from the skeletal muscle of 3-, 12- and 24-month-old Fischer 344 rats. In vitro growth of these cells was evaluated in serum-containing medium and in serum-free medium in response to multiplication stimulating activity/rat insulin-like growth factor II (MSA). Cells from 3-month-old rats exhibited a shorter lag phase of growth than cells from 12- or 24-month-old animals. Dose-response curves for MSA with each of the three age groups did not differ in the concentrations of MSA required for a half-maximal response or in the magnitudes of the response. Hormone-binding data using [¹²⁵I]MSA, however, revealed the highest numbers of MSA-binding sites with lowest affinities in the 3-month-old rat muscle cells; cells from the 24-month-old rats were intermediate and cells from the 12-month-old rats had the highest affinity and lowest number of binding sites. The lower affinity and increased number of binding sites in the young rat cells may be due to greater numbers of IGF type I receptors in muscle from young growing rats.

Original language	English (US)
Pages (from-to)	121-128
Number of pages	8
Journal	Mechanisms of Ageing and Development
Volume	39
Issue number	2
DOIs	https://doi.org/10.1016/0047-6374(87)90003-0
State	Published - Jul 1987

Keywords

Aging
Insulin-like growth factor
Muscle

ASJC Scopus subject areas

Aging
Developmental Biology

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10.1016/0047-6374(87)90003-0

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title = "Interaction of multiplication stimulating activity/rat insulin-like growth factor II with skeletal muscle satellite cells during aging",

abstract = "Satellite cells were isolated from the skeletal muscle of 3-, 12- and 24-month-old Fischer 344 rats. In vitro growth of these cells was evaluated in serum-containing medium and in serum-free medium in response to multiplication stimulating activity/rat insulin-like growth factor II (MSA). Cells from 3-month-old rats exhibited a shorter lag phase of growth than cells from 12- or 24-month-old animals. Dose-response curves for MSA with each of the three age groups did not differ in the concentrations of MSA required for a half-maximal response or in the magnitudes of the response. Hormone-binding data using [125I]MSA, however, revealed the highest numbers of MSA-binding sites with lowest affinities in the 3-month-old rat muscle cells; cells from the 24-month-old rats were intermediate and cells from the 12-month-old rats had the highest affinity and lowest number of binding sites. The lower affinity and increased number of binding sites in the young rat cells may be due to greater numbers of IGF type I receptors in muscle from young growing rats.",

keywords = "Aging, Insulin-like growth factor, Muscle",

author = "Dodson, {Michael V.} and Allen, {Ronald E.}",

note = "Funding Information: A reduction in skeletal muscle mass often accompanies aging, and this change may have an effect on gross motor activity as well as body protein metabolism. Specific causes underlying senile muscle atrophy have not been thoroughly defined, although observed morphological changes include fiber loss \[1\] as well as reductions in fiber *This paper is Arizona Agriculture Experiment Station Journal Paper No. 4224. The work was supported by Arizona Agriculture Experiment Station Project Rll and USPHS Grant R01 03393. **Present address: Department of Animal Science, Washington State University, Pullman, WA U.S.A. ***To whom all correspondence should be sent.",

year = "1987",

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T1 - Interaction of multiplication stimulating activity/rat insulin-like growth factor II with skeletal muscle satellite cells during aging

AU - Dodson, Michael V.

AU - Allen, Ronald E.

N1 - Funding Information: A reduction in skeletal muscle mass often accompanies aging, and this change may have an effect on gross motor activity as well as body protein metabolism. Specific causes underlying senile muscle atrophy have not been thoroughly defined, although observed morphological changes include fiber loss \[1\] as well as reductions in fiber *This paper is Arizona Agriculture Experiment Station Journal Paper No. 4224. The work was supported by Arizona Agriculture Experiment Station Project Rll and USPHS Grant R01 03393. **Present address: Department of Animal Science, Washington State University, Pullman, WA U.S.A. ***To whom all correspondence should be sent.

PY - 1987/7

Y1 - 1987/7

N2 - Satellite cells were isolated from the skeletal muscle of 3-, 12- and 24-month-old Fischer 344 rats. In vitro growth of these cells was evaluated in serum-containing medium and in serum-free medium in response to multiplication stimulating activity/rat insulin-like growth factor II (MSA). Cells from 3-month-old rats exhibited a shorter lag phase of growth than cells from 12- or 24-month-old animals. Dose-response curves for MSA with each of the three age groups did not differ in the concentrations of MSA required for a half-maximal response or in the magnitudes of the response. Hormone-binding data using [125I]MSA, however, revealed the highest numbers of MSA-binding sites with lowest affinities in the 3-month-old rat muscle cells; cells from the 24-month-old rats were intermediate and cells from the 12-month-old rats had the highest affinity and lowest number of binding sites. The lower affinity and increased number of binding sites in the young rat cells may be due to greater numbers of IGF type I receptors in muscle from young growing rats.

AB - Satellite cells were isolated from the skeletal muscle of 3-, 12- and 24-month-old Fischer 344 rats. In vitro growth of these cells was evaluated in serum-containing medium and in serum-free medium in response to multiplication stimulating activity/rat insulin-like growth factor II (MSA). Cells from 3-month-old rats exhibited a shorter lag phase of growth than cells from 12- or 24-month-old animals. Dose-response curves for MSA with each of the three age groups did not differ in the concentrations of MSA required for a half-maximal response or in the magnitudes of the response. Hormone-binding data using [125I]MSA, however, revealed the highest numbers of MSA-binding sites with lowest affinities in the 3-month-old rat muscle cells; cells from the 24-month-old rats were intermediate and cells from the 12-month-old rats had the highest affinity and lowest number of binding sites. The lower affinity and increased number of binding sites in the young rat cells may be due to greater numbers of IGF type I receptors in muscle from young growing rats.

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Interaction of multiplication stimulating activity/rat insulin-like growth factor II with skeletal muscle satellite cells during aging

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