Interaction of cortactin and Arp2/3 complex is required for sphingosine-1-phosphate-induced endothelial cell remodeling

Yansong Li, Takehito Uruno, Christian Haudenschild, Steven M. Dudek, Joe G.N. Garcia, Xi Zhan

Research output: Contribution to journalArticlepeer-review

30 Scopus citations


Sphingosine-1-phosphate (S1P) induces capillary formation of endothelial cells on Matrigel in accompany with actin assembly and accumulation of cortactin and Arp2/3 complex at the cell-leading edge. Suppression of cortactin expression with a cortactin antisense oligo significantly impaired S1P-induced capillary formation, migration of endothelial cells, and actin assembly at the cell periphery. Overexpression of wild-type cortactin tagged by green fluorescent protein (GFP) increased the S1P-induced tube formation and cell motility, whereas the cells overexpressing the mutant formed poorly capillary network and became less motile in response to S1P. Analysis of distribution in Triton X-100 insoluble fractions demonstrated that the cortactin mutant inhibited the association of wild-type cortactin and Arp2/3 complex with the actin-enriched complex. Furthermore, actin polymerization at and distribution of Arp2/3 complex as well as endogenous cortactin into the cell-leading edge mediated by S1P was disturbed. These data suggest that the interaction between cortactin and Arp2/3 complex plays an important role in S1P-mediated remodeling of endothelial cells.

Original languageEnglish (US)
Pages (from-to)107-121
Number of pages15
JournalExperimental Cell Research
Issue number1
StatePublished - Aug 1 2004


  • Actin cytoskeleton
  • Angiogenesis
  • Arp2/3 complex
  • Cortactin
  • EDG
  • Endothelial cell
  • FACS
  • HUVE
  • MLCK
  • Migration
  • S1P
  • endothelial differentiation gene
  • fluorescence-activated cell sorting
  • human umbilical vein endothelial
  • myosin light chain kinase

ASJC Scopus subject areas

  • Cell Biology


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