Interaction of cortactin and Arp2/3 complex is required for sphingosine-1-phosphate-induced endothelial cell remodeling

Yansong Li; Takehito Uruno; Christian Haudenschild; Steven M. Dudek; Joe G.N. Garcia; Xi Zhan

doi:10.1016/j.yexcr.2004.03.023

Interaction of cortactin and Arp2/3 complex is required for sphingosine-1-phosphate-induced endothelial cell remodeling

Yansong Li, Takehito Uruno, Christian Haudenschild, Steven M. Dudek, Joe G.N. Garcia, Xi Zhan

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

Sphingosine-1-phosphate (S1P) induces capillary formation of endothelial cells on Matrigel in accompany with actin assembly and accumulation of cortactin and Arp2/3 complex at the cell-leading edge. Suppression of cortactin expression with a cortactin antisense oligo significantly impaired S1P-induced capillary formation, migration of endothelial cells, and actin assembly at the cell periphery. Overexpression of wild-type cortactin tagged by green fluorescent protein (GFP) increased the S1P-induced tube formation and cell motility, whereas the cells overexpressing the mutant formed poorly capillary network and became less motile in response to S1P. Analysis of distribution in Triton X-100 insoluble fractions demonstrated that the cortactin mutant inhibited the association of wild-type cortactin and Arp2/3 complex with the actin-enriched complex. Furthermore, actin polymerization at and distribution of Arp2/3 complex as well as endogenous cortactin into the cell-leading edge mediated by S1P was disturbed. These data suggest that the interaction between cortactin and Arp2/3 complex plays an important role in S1P-mediated remodeling of endothelial cells.

Original language	English (US)
Pages (from-to)	107-121
Number of pages	15
Journal	Experimental Cell Research
Volume	298
Issue number	1
DOIs	https://doi.org/10.1016/j.yexcr.2004.03.023
State	Published - Aug 1 2004

Keywords

Actin cytoskeleton
Angiogenesis
Arp2/3 complex
Cortactin
EDG
Endothelial cell
FACS
HUVE
MLCK
Migration
S1P
endothelial differentiation gene
fluorescence-activated cell sorting
human umbilical vein endothelial
myosin light chain kinase

ASJC Scopus subject areas

Cell Biology

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10.1016/j.yexcr.2004.03.023

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@article{262bf566bba94d24a8edf123f129363f,

title = "Interaction of cortactin and Arp2/3 complex is required for sphingosine-1-phosphate-induced endothelial cell remodeling",

abstract = "Sphingosine-1-phosphate (S1P) induces capillary formation of endothelial cells on Matrigel in accompany with actin assembly and accumulation of cortactin and Arp2/3 complex at the cell-leading edge. Suppression of cortactin expression with a cortactin antisense oligo significantly impaired S1P-induced capillary formation, migration of endothelial cells, and actin assembly at the cell periphery. Overexpression of wild-type cortactin tagged by green fluorescent protein (GFP) increased the S1P-induced tube formation and cell motility, whereas the cells overexpressing the mutant formed poorly capillary network and became less motile in response to S1P. Analysis of distribution in Triton X-100 insoluble fractions demonstrated that the cortactin mutant inhibited the association of wild-type cortactin and Arp2/3 complex with the actin-enriched complex. Furthermore, actin polymerization at and distribution of Arp2/3 complex as well as endogenous cortactin into the cell-leading edge mediated by S1P was disturbed. These data suggest that the interaction between cortactin and Arp2/3 complex plays an important role in S1P-mediated remodeling of endothelial cells.",

keywords = "Actin cytoskeleton, Angiogenesis, Arp2/3 complex, Cortactin, EDG, Endothelial cell, FACS, HUVE, MLCK, Migration, S1P, endothelial differentiation gene, fluorescence-activated cell sorting, human umbilical vein endothelial, myosin light chain kinase",

author = "Yansong Li and Takehito Uruno and Christian Haudenschild and Dudek, {Steven M.} and Garcia, {Joe G.N.} and Xi Zhan",

note = "Funding Information: We thank Dr. Richard C. Mulligan (Harvard Medical School, Boston, MA) for providing 293GPG packaging cells, Dr. Jian-jiang Hao and Kang Zhou for helpful discussions with this work. We also gratefully acknowledge Ms Jiali Liu and Ms Nicole Smith for technical assistance. This work was supported by NIH grant R01 HL 52753-09, R01 CA 91984-01, DOD grant DAMD 17-01-1-0125, American Heart Association grant 0040135N (XZ), and P01 HL 58064, R01 68071, and R01 50533 (JGNG). SMD received support from the Four Schools Physician-Scientist Program, NRSA HL 10403, and NIH KO8 HL70013-01.",

year = "2004",

month = aug,

day = "1",

doi = "10.1016/j.yexcr.2004.03.023",

language = "English (US)",

volume = "298",

pages = "107--121",

journal = "Experimental Cell Research",

issn = "0014-4827",

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TY - JOUR

T1 - Interaction of cortactin and Arp2/3 complex is required for sphingosine-1-phosphate-induced endothelial cell remodeling

AU - Li, Yansong

AU - Uruno, Takehito

AU - Haudenschild, Christian

AU - Dudek, Steven M.

AU - Garcia, Joe G.N.

AU - Zhan, Xi

N1 - Funding Information: We thank Dr. Richard C. Mulligan (Harvard Medical School, Boston, MA) for providing 293GPG packaging cells, Dr. Jian-jiang Hao and Kang Zhou for helpful discussions with this work. We also gratefully acknowledge Ms Jiali Liu and Ms Nicole Smith for technical assistance. This work was supported by NIH grant R01 HL 52753-09, R01 CA 91984-01, DOD grant DAMD 17-01-1-0125, American Heart Association grant 0040135N (XZ), and P01 HL 58064, R01 68071, and R01 50533 (JGNG). SMD received support from the Four Schools Physician-Scientist Program, NRSA HL 10403, and NIH KO8 HL70013-01.

PY - 2004/8/1

Y1 - 2004/8/1

N2 - Sphingosine-1-phosphate (S1P) induces capillary formation of endothelial cells on Matrigel in accompany with actin assembly and accumulation of cortactin and Arp2/3 complex at the cell-leading edge. Suppression of cortactin expression with a cortactin antisense oligo significantly impaired S1P-induced capillary formation, migration of endothelial cells, and actin assembly at the cell periphery. Overexpression of wild-type cortactin tagged by green fluorescent protein (GFP) increased the S1P-induced tube formation and cell motility, whereas the cells overexpressing the mutant formed poorly capillary network and became less motile in response to S1P. Analysis of distribution in Triton X-100 insoluble fractions demonstrated that the cortactin mutant inhibited the association of wild-type cortactin and Arp2/3 complex with the actin-enriched complex. Furthermore, actin polymerization at and distribution of Arp2/3 complex as well as endogenous cortactin into the cell-leading edge mediated by S1P was disturbed. These data suggest that the interaction between cortactin and Arp2/3 complex plays an important role in S1P-mediated remodeling of endothelial cells.

AB - Sphingosine-1-phosphate (S1P) induces capillary formation of endothelial cells on Matrigel in accompany with actin assembly and accumulation of cortactin and Arp2/3 complex at the cell-leading edge. Suppression of cortactin expression with a cortactin antisense oligo significantly impaired S1P-induced capillary formation, migration of endothelial cells, and actin assembly at the cell periphery. Overexpression of wild-type cortactin tagged by green fluorescent protein (GFP) increased the S1P-induced tube formation and cell motility, whereas the cells overexpressing the mutant formed poorly capillary network and became less motile in response to S1P. Analysis of distribution in Triton X-100 insoluble fractions demonstrated that the cortactin mutant inhibited the association of wild-type cortactin and Arp2/3 complex with the actin-enriched complex. Furthermore, actin polymerization at and distribution of Arp2/3 complex as well as endogenous cortactin into the cell-leading edge mediated by S1P was disturbed. These data suggest that the interaction between cortactin and Arp2/3 complex plays an important role in S1P-mediated remodeling of endothelial cells.

KW - Actin cytoskeleton

KW - Angiogenesis

KW - Arp2/3 complex

KW - Cortactin

KW - EDG

KW - Endothelial cell

KW - FACS

KW - HUVE

KW - MLCK

KW - Migration

KW - S1P

KW - endothelial differentiation gene

KW - fluorescence-activated cell sorting

KW - human umbilical vein endothelial

KW - myosin light chain kinase

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UR - http://www.scopus.com/inward/citedby.url?scp=3042820790&partnerID=8YFLogxK

U2 - 10.1016/j.yexcr.2004.03.023

DO - 10.1016/j.yexcr.2004.03.023

M3 - Article

C2 - 15242766

AN - SCOPUS:3042820790

VL - 298

SP - 107

EP - 121

JO - Experimental Cell Research

JF - Experimental Cell Research

SN - 0014-4827

IS - 1

ER -

Interaction of cortactin and Arp2/3 complex is required for sphingosine-1-phosphate-induced endothelial cell remodeling

Abstract

Keywords

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