TY - JOUR
T1 - Integrated microfluidic system for rapid forensic dna analysis
T2 - Sample collection to DNA Profile
AU - Hopwood, Andrew J.
AU - Hurth, Cedric
AU - Yang, Jianing
AU - Cai, Zhi
AU - Moran, Nina
AU - Lee-Edghill, John G.
AU - Nordquist, Alan
AU - Lenigk, Ralf
AU - Estes, Matthew D.
AU - Haley, John P.
AU - McAlister, Colin R.
AU - Chen, Xiaojia
AU - Brooks, Carla
AU - Smith, Stan
AU - Elliott, Keith
AU - Koumi, Pieris
AU - Zenhausern, Frederic
AU - Tully, Gillian
PY - 2010/8/15
Y1 - 2010/8/15
N2 - We demonstrate a conduit for the delivery of a step change in the DNA analysis process: A fully integrated instrument for the analysis of multiplex short tandem repeat DNA profiles from reference buccal samples is described and is suitable for the processing of such samples within a forensic environment such as a police custody suite or booking office. The instrument is loaded with a DNA processing cartridge which incorporates on-board pumps and valves which direct the delivery of sample and reagents to the various reaction chambers to allow DNA purification, amplification of the DNA by PCR, and collection of the amplified product for delivery to an integral CE chip. The fluorescently labeled product is separated using micro capillary electrophoresis with a resolution of 1.2 base pairs (bp) allowing laser induced fluorescence-based detection of the amplified short tandem repeat fragments and subsequent analysis of data to produce a DNA profile which is compatible with the data format of the UK DNA database. The entire process from taking the sample from a suspect, to database compatible DNA profile production can currently be achieved in less than 4 h. By integrating such an instrument and microfluidic cartridge with the forensic process, we believe it will be possible in the near future to process a DNA sample taken from an individual in police custody and compare the profile with the DNA profiles held on a DNA Database in as little as 3 h.
AB - We demonstrate a conduit for the delivery of a step change in the DNA analysis process: A fully integrated instrument for the analysis of multiplex short tandem repeat DNA profiles from reference buccal samples is described and is suitable for the processing of such samples within a forensic environment such as a police custody suite or booking office. The instrument is loaded with a DNA processing cartridge which incorporates on-board pumps and valves which direct the delivery of sample and reagents to the various reaction chambers to allow DNA purification, amplification of the DNA by PCR, and collection of the amplified product for delivery to an integral CE chip. The fluorescently labeled product is separated using micro capillary electrophoresis with a resolution of 1.2 base pairs (bp) allowing laser induced fluorescence-based detection of the amplified short tandem repeat fragments and subsequent analysis of data to produce a DNA profile which is compatible with the data format of the UK DNA database. The entire process from taking the sample from a suspect, to database compatible DNA profile production can currently be achieved in less than 4 h. By integrating such an instrument and microfluidic cartridge with the forensic process, we believe it will be possible in the near future to process a DNA sample taken from an individual in police custody and compare the profile with the DNA profiles held on a DNA Database in as little as 3 h.
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U2 - 10.1021/ac101355r
DO - 10.1021/ac101355r
M3 - Article
C2 - 20704389
AN - SCOPUS:77955612070
VL - 82
SP - 6991
EP - 6999
JO - Analytical Chemistry
JF - Analytical Chemistry
SN - 0003-2700
IS - 16
ER -