Integrated bioprinting and imaging for scalable, networkable desktop experimentation

Nathan D. Orloff, Cynthia Truong, Nathan Cira, Stephen Koo, Andrea Hamilton, Sean Choi, Victoria Wu, Ingmar H. Riedel-Kruse

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Adaptations of mass-market consumer electronics are increasingly used to aid experimentation in engineering, life sciences, and education. Inspired by recent bioprinting and imaging research, we have developed a low-cost, networkable, scalable bioprinter with integrated imaging that enables automated fluid deposition with monitoring biological materials over a large area that can interface with standard plasticware. We re-engineered a desktop printer and scanner with mechanical workarounds (rather than software) to leverage the unmodified software, creating a complete system for ∼$700. We found that the resulting print accuracy and precision of this system were close to those of the unmodified printer and scanner. We demonstrate the reach of this system by printing multiple strains of Escherichia coli, performing quantitative time-lapse recordings of bacterial growth, and then separating different fluorescent strains according to color. A fluid-deposition and imaging platform, like the one developed here, could be integrated for do-it-yourself research, remote experimentation, and (on-line) education. This journal is

Original languageEnglish (US)
Pages (from-to)34721-34728
Number of pages8
JournalRSC Advances
Volume4
Issue number65
DOIs
StatePublished - 2014
Externally publishedYes

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)

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