Insights into the chemical biology of selenium

Richard S. Glass; Marla J. Berry; Eric Block; Harriet T. Boakye; Bradley A. Carlson; Jrgen Gailer; Graham N. George; Vadim N. Gladyshev; Dolph L. Hatfield; Neil E. Jacobsen; Sherida Johnson; Chethaka Kahakachchi; Rafal Kaminski; Shawn A. Manley; Heiko Mix; Ingrid J. Pickering; Elmar J. Prenner; Kazima Saira; Aleksandra Skowronska; Julian F. Tyson; Peter C. Uden; Qiao Wu; Xue Ming Xu; Raghav Yamdagni; Yan Zhang

doi:10.1080/10426500801898366

Insights into the chemical biology of selenium

Richard S. Glass, Marla J. Berry, Eric Block, Harriet T. Boakye, Bradley A. Carlson, Jrgen Gailer, Graham N. George, Vadim N. Gladyshev, Dolph L. Hatfield, Neil E. Jacobsen, Sherida Johnson, Chethaka Kahakachchi, Rafal Kaminski, Shawn A. Manley, Heiko Mix, Ingrid J. Pickering, Elmar J. Prenner, Kazima Saira, Aleksandra Skowronska, Julian F. TysonPeter C. Uden, Qiao Wu, Xue Ming Xu, Raghav Yamdagni, Yan Zhang

Chemistry and Biochemistry

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

The long-sought pathway by which selenocysteyl-tRNA[Ser]Sec is synthesized in eukaryotes has been revealed. Seryl-tRNA[Ser]Sec is O-phosphorylated and SecS, a pyridoxal phosphate-dependent protein, catalyzes the reaction of O-phosphoseryl-tRNA[Ser]Sec with monoselenophosphate to give selenocysteyl-tRNA[Ser]Sec. 1H-77Se HMQC-TOCSY NMR spectroscopy has been developed to detect the selenium-containing amino acids present in selenized yeast after protease XIV digestion. An archived selenized yeast sample is found to contain the novel amino acid S-(methylseleno)cysteine in addition to selenomethionine. Arsenite and selenite react with GSH to form (GS)2AsSe-. The structure of this compound has been determined by EXAFS, 77Se NMR and Raman spectroscopic and chromatographic studies. Its formation under biological conditions has been demonstrated.

Original language	English (US)
Pages (from-to)	924-930
Number of pages	7
Journal	Phosphorus, Sulfur and Silicon and the Related Elements
Volume	183
Issue number	4
DOIs	https://doi.org/10.1080/10426500801898366
State	Published - Apr 2008

ASJC Scopus subject areas

Biochemistry
Organic Chemistry
Inorganic Chemistry

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10.1080/10426500801898366

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Glass, R. S., Berry, M. J., Block, E., Boakye, H. T., Carlson, B. A., Gailer, J., George, G. N., Gladyshev, V. N., Hatfield, D. L., Jacobsen, N. E., Johnson, S., Kahakachchi, C., Kaminski, R., Manley, S. A., Mix, H., Pickering, I. J., Prenner, E. J., Saira, K., Skowronska, A., ... Zhang, Y. (2008). Insights into the chemical biology of selenium. Phosphorus, Sulfur and Silicon and the Related Elements, 183(4), 924-930. https://doi.org/10.1080/10426500801898366

Glass, RS, Berry, MJ, Block, E, Boakye, HT, Carlson, BA, Gailer, J, George, GN, Gladyshev, VN, Hatfield, DL, Jacobsen, NE, Johnson, S, Kahakachchi, C, Kaminski, R, Manley, SA, Mix, H, Pickering, IJ, Prenner, EJ, Saira, K, Skowronska, A, Tyson, JF, Uden, PC, Wu, Q, Xu, XM, Yamdagni, R & Zhang, Y 2008, 'Insights into the chemical biology of selenium', Phosphorus, Sulfur and Silicon and the Related Elements, vol. 183, no. 4, pp. 924-930. https://doi.org/10.1080/10426500801898366

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title = "Insights into the chemical biology of selenium",

abstract = "The long-sought pathway by which selenocysteyl-tRNA[Ser]Sec is synthesized in eukaryotes has been revealed. Seryl-tRNA[Ser]Sec is O-phosphorylated and SecS, a pyridoxal phosphate-dependent protein, catalyzes the reaction of O-phosphoseryl-tRNA[Ser]Sec with monoselenophosphate to give selenocysteyl-tRNA[Ser]Sec. 1H-77Se HMQC-TOCSY NMR spectroscopy has been developed to detect the selenium-containing amino acids present in selenized yeast after protease XIV digestion. An archived selenized yeast sample is found to contain the novel amino acid S-(methylseleno)cysteine in addition to selenomethionine. Arsenite and selenite react with GSH to form (GS)2AsSe-. The structure of this compound has been determined by EXAFS, 77Se NMR and Raman spectroscopic and chromatographic studies. Its formation under biological conditions has been demonstrated.",

author = "Glass, {Richard S.} and Berry, {Marla J.} and Eric Block and Boakye, {Harriet T.} and Carlson, {Bradley A.} and Jrgen Gailer and George, {Graham N.} and Gladyshev, {Vadim N.} and Hatfield, {Dolph L.} and Jacobsen, {Neil E.} and Sherida Johnson and Chethaka Kahakachchi and Rafal Kaminski and Manley, {Shawn A.} and Heiko Mix and Pickering, {Ingrid J.} and Prenner, {Elmar J.} and Kazima Saira and Aleksandra Skowronska and Tyson, {Julian F.} and Uden, {Peter C.} and Qiao Wu and Xu, {Xue Ming} and Raghav Yamdagni and Yan Zhang",

note = "Funding Information: The support of this research by the U.S. National Science Foundation, the U.S. National Institutes of Health, and the Petroleum Research Fund, administered by the American Chemical Society, is gratefully acknowledged.",

year = "2008",

month = apr,

doi = "10.1080/10426500801898366",

language = "English (US)",

volume = "183",

pages = "924--930",

journal = "Phosphorus, Sulfur and Silicon and the Related Elements",

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T1 - Insights into the chemical biology of selenium

AU - Glass, Richard S.

AU - Berry, Marla J.

AU - Block, Eric

AU - Boakye, Harriet T.

AU - Carlson, Bradley A.

AU - Gailer, Jrgen

AU - George, Graham N.

AU - Gladyshev, Vadim N.

AU - Hatfield, Dolph L.

AU - Jacobsen, Neil E.

AU - Johnson, Sherida

AU - Kahakachchi, Chethaka

AU - Kaminski, Rafal

AU - Manley, Shawn A.

AU - Mix, Heiko

AU - Pickering, Ingrid J.

AU - Prenner, Elmar J.

AU - Saira, Kazima

AU - Skowronska, Aleksandra

AU - Tyson, Julian F.

AU - Uden, Peter C.

AU - Wu, Qiao

AU - Xu, Xue Ming

AU - Yamdagni, Raghav

AU - Zhang, Yan

N1 - Funding Information: The support of this research by the U.S. National Science Foundation, the U.S. National Institutes of Health, and the Petroleum Research Fund, administered by the American Chemical Society, is gratefully acknowledged.

PY - 2008/4

Y1 - 2008/4

N2 - The long-sought pathway by which selenocysteyl-tRNA[Ser]Sec is synthesized in eukaryotes has been revealed. Seryl-tRNA[Ser]Sec is O-phosphorylated and SecS, a pyridoxal phosphate-dependent protein, catalyzes the reaction of O-phosphoseryl-tRNA[Ser]Sec with monoselenophosphate to give selenocysteyl-tRNA[Ser]Sec. 1H-77Se HMQC-TOCSY NMR spectroscopy has been developed to detect the selenium-containing amino acids present in selenized yeast after protease XIV digestion. An archived selenized yeast sample is found to contain the novel amino acid S-(methylseleno)cysteine in addition to selenomethionine. Arsenite and selenite react with GSH to form (GS)2AsSe-. The structure of this compound has been determined by EXAFS, 77Se NMR and Raman spectroscopic and chromatographic studies. Its formation under biological conditions has been demonstrated.

AB - The long-sought pathway by which selenocysteyl-tRNA[Ser]Sec is synthesized in eukaryotes has been revealed. Seryl-tRNA[Ser]Sec is O-phosphorylated and SecS, a pyridoxal phosphate-dependent protein, catalyzes the reaction of O-phosphoseryl-tRNA[Ser]Sec with monoselenophosphate to give selenocysteyl-tRNA[Ser]Sec. 1H-77Se HMQC-TOCSY NMR spectroscopy has been developed to detect the selenium-containing amino acids present in selenized yeast after protease XIV digestion. An archived selenized yeast sample is found to contain the novel amino acid S-(methylseleno)cysteine in addition to selenomethionine. Arsenite and selenite react with GSH to form (GS)2AsSe-. The structure of this compound has been determined by EXAFS, 77Se NMR and Raman spectroscopic and chromatographic studies. Its formation under biological conditions has been demonstrated.

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JF - Phosphorus, Sulfur and Silicon and the Related Elements

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ER -

Insights into the chemical biology of selenium

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