Inositol 1,4,5-trisphosphate releases Ca2+ from vacuolar membrane vesicles of oat roots.

K. S. Schumaker; H. Sze

doi:10.1016/s0021-9258(18)61292-x

Inositol 1,4,5-trisphosphate releases Ca2+ from vacuolar membrane vesicles of oat roots.

K. S. Schumaker, H. Sze

Research output: Contribution to journal › Article › peer-review

202 Scopus citations

Abstract

In plant cells, transient changes in cytoplasmic Ca2+ levels can modulate numerous developmental processes. Ca2+ is accumulated in the vacuole via a H+/Ca2+ antiport system that is energized by the tonoplast H+-pumping ATPase. Inositol 1,4,5-triphosphate (InsP3), but not inositol 1,4-bisphosphate, myo-inositol 1-phosphate, or fructose 2,6-bisphosphate, caused a transient reduction of Ca2+ levels in tonoplast vesicles. The decrease was dependent on InsP3 concentration (Km apparent = 0.6 microM). The InsP3-induced Ca2+ release was blocked by the Ca2+ antagonist, 8-(N,N-diethylamino)-octyl 3,4,5-trimethoxybenzoate-HCl. These results suggest that the vacuolar membrane is one target site for InsP3 action and that InsP3 may operate as a second messenger in the mobilization of intracellular Ca2+ in plant cells.

Original language	English (US)
Pages (from-to)	3944-3946
Number of pages	3
Journal	The Journal of biological chemistry
Volume	262
Issue number	9
DOIs	https://doi.org/10.1016/s0021-9258(18)61292-x
State	Published - Mar 25 1987
Externally published	Yes

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Cell Biology

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10.1016/s0021-9258(18)61292-x

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title = "Inositol 1,4,5-trisphosphate releases Ca2+ from vacuolar membrane vesicles of oat roots.",

abstract = "In plant cells, transient changes in cytoplasmic Ca2+ levels can modulate numerous developmental processes. Ca2+ is accumulated in the vacuole via a H+/Ca2+ antiport system that is energized by the tonoplast H+-pumping ATPase. Inositol 1,4,5-triphosphate (InsP3), but not inositol 1,4-bisphosphate, myo-inositol 1-phosphate, or fructose 2,6-bisphosphate, caused a transient reduction of Ca2+ levels in tonoplast vesicles. The decrease was dependent on InsP3 concentration (Km apparent = 0.6 microM). The InsP3-induced Ca2+ release was blocked by the Ca2+ antagonist, 8-(N,N-diethylamino)-octyl 3,4,5-trimethoxybenzoate-HCl. These results suggest that the vacuolar membrane is one target site for InsP3 action and that InsP3 may operate as a second messenger in the mobilization of intracellular Ca2+ in plant cells.",

author = "Schumaker, \{K. S.\} and H. Sze",

year = "1987",

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doi = "10.1016/s0021-9258(18)61292-x",

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journal = "The Journal of biological chemistry",

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T1 - Inositol 1,4,5-trisphosphate releases Ca2+ from vacuolar membrane vesicles of oat roots.

AU - Schumaker, K. S.

AU - Sze, H.

PY - 1987/3/25

Y1 - 1987/3/25

N2 - In plant cells, transient changes in cytoplasmic Ca2+ levels can modulate numerous developmental processes. Ca2+ is accumulated in the vacuole via a H+/Ca2+ antiport system that is energized by the tonoplast H+-pumping ATPase. Inositol 1,4,5-triphosphate (InsP3), but not inositol 1,4-bisphosphate, myo-inositol 1-phosphate, or fructose 2,6-bisphosphate, caused a transient reduction of Ca2+ levels in tonoplast vesicles. The decrease was dependent on InsP3 concentration (Km apparent = 0.6 microM). The InsP3-induced Ca2+ release was blocked by the Ca2+ antagonist, 8-(N,N-diethylamino)-octyl 3,4,5-trimethoxybenzoate-HCl. These results suggest that the vacuolar membrane is one target site for InsP3 action and that InsP3 may operate as a second messenger in the mobilization of intracellular Ca2+ in plant cells.

AB - In plant cells, transient changes in cytoplasmic Ca2+ levels can modulate numerous developmental processes. Ca2+ is accumulated in the vacuole via a H+/Ca2+ antiport system that is energized by the tonoplast H+-pumping ATPase. Inositol 1,4,5-triphosphate (InsP3), but not inositol 1,4-bisphosphate, myo-inositol 1-phosphate, or fructose 2,6-bisphosphate, caused a transient reduction of Ca2+ levels in tonoplast vesicles. The decrease was dependent on InsP3 concentration (Km apparent = 0.6 microM). The InsP3-induced Ca2+ release was blocked by the Ca2+ antagonist, 8-(N,N-diethylamino)-octyl 3,4,5-trimethoxybenzoate-HCl. These results suggest that the vacuolar membrane is one target site for InsP3 action and that InsP3 may operate as a second messenger in the mobilization of intracellular Ca2+ in plant cells.

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U2 - 10.1016/s0021-9258(18)61292-x

DO - 10.1016/s0021-9258(18)61292-x

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AN - SCOPUS:0023664149

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JF - The Journal of biological chemistry

IS - 9

ER -

Inositol 1,4,5-trisphosphate releases Ca2+ from vacuolar membrane vesicles of oat roots.

Abstract

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