Initiation of puberty in mice following decellularized ovary transplant

Monica M. Laronda, Adam E. Jakus, Kelly A. Whelan, Jason A. Wertheim, Ramille N. Shah, Teresa K. Woodruff

Research output: Contribution to journalArticlepeer-review

146 Scopus citations


Clinical interventions to preserve fertility and restore hormone levels in female patients with therapy-induced ovarian failure are insufficient, particularly for pediatric cancer patients. Laparoscopic isolation of cortical ovarian tissue followed by cryopreservation with subsequent autotransplantation has temporarily restored fertility in at least 27 women who survived cancer, and aided in pubertal transition for one pediatric patient. However, reintroducing cancer cells through ovarian transplantation has been a major concern. Decellularization is a process of removing cellular material, while maintaining the organ skeleton of extracellular matrices (ECM). The ECM that remains could be stripped of cancer cells and reseeded with healthy ovarian cells. We tested whether a decellularized ovarian scaffold could be created, recellularized and transplanted to initiate puberty in mice. Bovine and human ovaries were decellularized, and the ovarian skeleton microstructures were characterized. Primary ovarian cells seeded onto decellularized scaffolds produced estradiol invitro. Moreover, the recellularized grafts initiated puberty in mice that had been ovariectomized, providing data that could be used to drive future human transplants and have broader implications on the bioengineering of other organs with endocrine function.

Original languageEnglish (US)
Pages (from-to)20-29
Number of pages10
Issue number1
StatePublished - 2015
Externally publishedYes


  • Bioactivity
  • Decellularization
  • ECM (extracellular matrix)
  • Endocrine function
  • SEM (scanning electron microscopy)
  • Xenotransplantation

ASJC Scopus subject areas

  • Biophysics
  • Bioengineering
  • Ceramics and Composites
  • Biomaterials
  • Mechanics of Materials


Dive into the research topics of 'Initiation of puberty in mice following decellularized ovary transplant'. Together they form a unique fingerprint.

Cite this