Infrared biosensors using hydrophobic chalcogenide fibers sensitized with live cells

Pierre Lucas; Michelle A. Solis; David Le Coq; Christophe Juncker; Mark R. Riley; Jayne Collier; Dianne E. Boesewetter; Catherine Boussard-Plédel; Bruno Bureau

doi:10.1016/j.snb.2005.12.033

Infrared biosensors using hydrophobic chalcogenide fibers sensitized with live cells

Pierre Lucas, Michelle A. Solis, David Le Coq, Christophe Juncker, Mark R. Riley, Jayne Collier, Dianne E. Boesewetter, Catherine Boussard-Plédel, Bruno Bureau

Research output: Contribution to journal › Article › peer-review

95 Scopus citations

Abstract

Chalcogenide glass fibers are used to perform remote infrared analysis in a fiber evanescent wave spectroscopy system (FEWS). Spectral analysis of a series of organic species demonstrates that the detection signal of non-polar species is enhanced by up to 60% relative to that of water. Contact angle measurements of the organic liquid/glass interaction strength shows that the spectral enhancement is the result of glass surface hydrophobicity. The greater interaction of non-polar species with the chalcogenide surface leads to a concentration gradient of the organic species at the glass surface where the evanescent wave is most intense. This results in selective detection of non-polar organic species in aqueous solution using FEWS. This behavior is beneficial for the detection of microorganism by FEWS and is used to design bio-optic sensors based on monitoring the response of live cells to toxins by detecting change in their infrared spectrum. This technique permits observation of disruption induced in living mammalian cells by at least two different types of toxicants. It is shown that it is possible to distinguish between the effect of a genotoxic agent (which damage nucleic acids) and a cytotoxic agent (which damages other cellular components) based on the cell's IR response.

Original language	English (US)
Pages (from-to)	355-362
Number of pages	8
Journal	Sensors and Actuators, B: Chemical
Volume	119
Issue number	2
DOIs	https://doi.org/10.1016/j.snb.2005.12.033
State	Published - Dec 7 2006

Keywords

Biosensor
Cell-based sensors
Chalcogenide fiber
Evanescent wave spectroscopy toxicity
FT-IR

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Instrumentation
Condensed Matter Physics
Surfaces, Coatings and Films
Metals and Alloys
Electrical and Electronic Engineering
Materials Chemistry

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10.1016/j.snb.2005.12.033

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title = "Infrared biosensors using hydrophobic chalcogenide fibers sensitized with live cells",

abstract = "Chalcogenide glass fibers are used to perform remote infrared analysis in a fiber evanescent wave spectroscopy system (FEWS). Spectral analysis of a series of organic species demonstrates that the detection signal of non-polar species is enhanced by up to 60% relative to that of water. Contact angle measurements of the organic liquid/glass interaction strength shows that the spectral enhancement is the result of glass surface hydrophobicity. The greater interaction of non-polar species with the chalcogenide surface leads to a concentration gradient of the organic species at the glass surface where the evanescent wave is most intense. This results in selective detection of non-polar organic species in aqueous solution using FEWS. This behavior is beneficial for the detection of microorganism by FEWS and is used to design bio-optic sensors based on monitoring the response of live cells to toxins by detecting change in their infrared spectrum. This technique permits observation of disruption induced in living mammalian cells by at least two different types of toxicants. It is shown that it is possible to distinguish between the effect of a genotoxic agent (which damage nucleic acids) and a cytotoxic agent (which damages other cellular components) based on the cell's IR response.",

keywords = "Biosensor, Cell-based sensors, Chalcogenide fiber, Evanescent wave spectroscopy toxicity, FT-IR",

author = "Pierre Lucas and Solis, {Michelle A.} and Coq, {David Le} and Christophe Juncker and Riley, {Mark R.} and Jayne Collier and Boesewetter, {Dianne E.} and Catherine Boussard-Pl{\'e}del and Bruno Bureau",

note = "Funding Information: This work was supported by DARPA Contract # N66001-C-8041, by the NIEHS sponsored Southwest Environmental Health Sciences Center # P30 ES06694, and by the Arizona Board of Regents Technology and Research Initiative Fund. ",

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doi = "10.1016/j.snb.2005.12.033",

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T1 - Infrared biosensors using hydrophobic chalcogenide fibers sensitized with live cells

AU - Lucas, Pierre

AU - Solis, Michelle A.

AU - Coq, David Le

AU - Juncker, Christophe

AU - Riley, Mark R.

AU - Collier, Jayne

AU - Boesewetter, Dianne E.

AU - Boussard-Plédel, Catherine

AU - Bureau, Bruno

N1 - Funding Information: This work was supported by DARPA Contract # N66001-C-8041, by the NIEHS sponsored Southwest Environmental Health Sciences Center # P30 ES06694, and by the Arizona Board of Regents Technology and Research Initiative Fund.

PY - 2006/12/7

Y1 - 2006/12/7

N2 - Chalcogenide glass fibers are used to perform remote infrared analysis in a fiber evanescent wave spectroscopy system (FEWS). Spectral analysis of a series of organic species demonstrates that the detection signal of non-polar species is enhanced by up to 60% relative to that of water. Contact angle measurements of the organic liquid/glass interaction strength shows that the spectral enhancement is the result of glass surface hydrophobicity. The greater interaction of non-polar species with the chalcogenide surface leads to a concentration gradient of the organic species at the glass surface where the evanescent wave is most intense. This results in selective detection of non-polar organic species in aqueous solution using FEWS. This behavior is beneficial for the detection of microorganism by FEWS and is used to design bio-optic sensors based on monitoring the response of live cells to toxins by detecting change in their infrared spectrum. This technique permits observation of disruption induced in living mammalian cells by at least two different types of toxicants. It is shown that it is possible to distinguish between the effect of a genotoxic agent (which damage nucleic acids) and a cytotoxic agent (which damages other cellular components) based on the cell's IR response.

AB - Chalcogenide glass fibers are used to perform remote infrared analysis in a fiber evanescent wave spectroscopy system (FEWS). Spectral analysis of a series of organic species demonstrates that the detection signal of non-polar species is enhanced by up to 60% relative to that of water. Contact angle measurements of the organic liquid/glass interaction strength shows that the spectral enhancement is the result of glass surface hydrophobicity. The greater interaction of non-polar species with the chalcogenide surface leads to a concentration gradient of the organic species at the glass surface where the evanescent wave is most intense. This results in selective detection of non-polar organic species in aqueous solution using FEWS. This behavior is beneficial for the detection of microorganism by FEWS and is used to design bio-optic sensors based on monitoring the response of live cells to toxins by detecting change in their infrared spectrum. This technique permits observation of disruption induced in living mammalian cells by at least two different types of toxicants. It is shown that it is possible to distinguish between the effect of a genotoxic agent (which damage nucleic acids) and a cytotoxic agent (which damages other cellular components) based on the cell's IR response.

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KW - Evanescent wave spectroscopy toxicity

KW - FT-IR

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ER -

Infrared biosensors using hydrophobic chalcogenide fibers sensitized with live cells

Abstract

Keywords

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