Influence of pH on the MI-MII equilibrium of rhodopsin in recombinant membranes

Nicholas J. Gibson; Michael F. Brown

doi:10.1016/0006-291X(90)91997-7

Influence of pH on the MI-MII equilibrium of rhodopsin in recombinant membranes

Nicholas J. Gibson, Michael F. Brown

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

Rhodopsin in native rod membranes and incorporated into egg phosphatidylcholine (egg PC) vesicles was studied at pH 5 and 7 at 28°C. Rhodopsin function, as monitored by the formation of metarhodopsin II (MII) from metarhodopsin I (MI) following an actinic flash, was found to be largely blocked in egg PC vesicles at pH 7. When the pH was lowered to 5, however, rhodopsin showed essentially equal activity in both native and egg PC membranes. This activity exceeded that found for rhodopsin in native membranes at pH 7. Phospholipid composition is thus shown to directly affect the MI ⇌ MII equilibrium, which in turn is linked to visual function.

Original language	English (US)
Pages (from-to)	1028-1034
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	169
Issue number	3
DOIs	https://doi.org/10.1016/0006-291X(90)91997-7
State	Published - Jun 29 1990

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/0006-291X(90)91997-7

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title = "Influence of pH on the MI-MII equilibrium of rhodopsin in recombinant membranes",

abstract = "Rhodopsin in native rod membranes and incorporated into egg phosphatidylcholine (egg PC) vesicles was studied at pH 5 and 7 at 28°C. Rhodopsin function, as monitored by the formation of metarhodopsin II (MII) from metarhodopsin I (MI) following an actinic flash, was found to be largely blocked in egg PC vesicles at pH 7. When the pH was lowered to 5, however, rhodopsin showed essentially equal activity in both native and egg PC membranes. This activity exceeded that found for rhodopsin in native membranes at pH 7. Phospholipid composition is thus shown to directly affect the MI ⇌ MII equilibrium, which in turn is linked to visual function.",

author = "Gibson, {Nicholas J.} and Brown, {Michael F.}",

note = "Funding Information: This work was supported by National Institutes of Health Grant EY03754. Thanks are due to Richard Schmidt for help with the design and construction of the PMT electronics and to Constantine Job and James Beach for helpful discussions. We and George Atkinson for critically reading the manuscript.",

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T1 - Influence of pH on the MI-MII equilibrium of rhodopsin in recombinant membranes

AU - Gibson, Nicholas J.

AU - Brown, Michael F.

N1 - Funding Information: This work was supported by National Institutes of Health Grant EY03754. Thanks are due to Richard Schmidt for help with the design and construction of the PMT electronics and to Constantine Job and James Beach for helpful discussions. We and George Atkinson for critically reading the manuscript.

PY - 1990/6/29

Y1 - 1990/6/29

N2 - Rhodopsin in native rod membranes and incorporated into egg phosphatidylcholine (egg PC) vesicles was studied at pH 5 and 7 at 28°C. Rhodopsin function, as monitored by the formation of metarhodopsin II (MII) from metarhodopsin I (MI) following an actinic flash, was found to be largely blocked in egg PC vesicles at pH 7. When the pH was lowered to 5, however, rhodopsin showed essentially equal activity in both native and egg PC membranes. This activity exceeded that found for rhodopsin in native membranes at pH 7. Phospholipid composition is thus shown to directly affect the MI ⇌ MII equilibrium, which in turn is linked to visual function.

AB - Rhodopsin in native rod membranes and incorporated into egg phosphatidylcholine (egg PC) vesicles was studied at pH 5 and 7 at 28°C. Rhodopsin function, as monitored by the formation of metarhodopsin II (MII) from metarhodopsin I (MI) following an actinic flash, was found to be largely blocked in egg PC vesicles at pH 7. When the pH was lowered to 5, however, rhodopsin showed essentially equal activity in both native and egg PC membranes. This activity exceeded that found for rhodopsin in native membranes at pH 7. Phospholipid composition is thus shown to directly affect the MI ⇌ MII equilibrium, which in turn is linked to visual function.

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Influence of pH on the MI-MII equilibrium of rhodopsin in recombinant membranes

Abstract

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