Infectious myonecrosis virus has a totivirus-like, 120-subunit capsid, but with fiber complexes at the fivefold axes

Jinghua Tang; Wendy F. Ochoa; Robert S. Sinkovits; Bonnie T. Poulos; Said A. Ghabrial; Donald V. Lightner; Timothy S. Baker; Max L. Nibert

doi:10.1073/pnas.0806724105

Infectious myonecrosis virus has a totivirus-like, 120-subunit capsid, but with fiber complexes at the fivefold axes

Jinghua Tang, Wendy F. Ochoa, Robert S. Sinkovits, Bonnie T. Poulos, Said A. Ghabrial, Donald V. Lightner, Timothy S. Baker, Max L. Nibert

Research output: Contribution to journal › Article › peer-review

52 Scopus citations

Abstract

Infectious myonecrosis virus (IMNV) is an emerging pathogen of penaeid shrimp in global aquaculture. Tentatively assigned to family Totiviridae, it has a nonsegmented dsRNA genome of 7,560 bp and an isometric capsid of the 901-aa major capsid protein. We used electron cryomicroscopy and 3D image reconstruction to examine the IMNV virion at 8.0-Å resolution. Results reveal a totivirus-like, 120-subunit T = 1 capsid, 450 Å in diameter, but with fiber complexes protruding a further 80 Å at the fivefold axes. These protrusions likely mediate roles in the extracellular transmission and pathogenesis of IMNV, capabilities not shared by most other totiviruses. The IMNV structure is also notable in that the genome is centrally organized in five or six concentric shells. Within each of these shells, the densities alternate between a dodecahedral frame that connects the threefold axes vs. concentration around the fivefold axes, implying certain regularities in the RNA packing scheme.

Original language	English (US)
Pages (from-to)	17526-17531
Number of pages	6
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	105
Issue number	45
DOIs	https://doi.org/10.1073/pnas.0806724105
State	Published - Nov 11 2008

Keywords

Electron cryomicroscopy
Nonenveloped virus
Penaeid shrimp
Totiviridae
dsRNA virus

ASJC Scopus subject areas

General

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10.1073/pnas.0806724105

Cite this

Tang, J., Ochoa, W. F., Sinkovits, R. S., Poulos, B. T., Ghabrial, S. A., Lightner, D. V., Baker, T. S., & Nibert, M. L. (2008). Infectious myonecrosis virus has a totivirus-like, 120-subunit capsid, but with fiber complexes at the fivefold axes. Proceedings of the National Academy of Sciences of the United States of America, 105(45), 17526-17531. https://doi.org/10.1073/pnas.0806724105

Infectious myonecrosis virus has a totivirus-like, 120-subunit capsid, but with fiber complexes at the fivefold axes. / Tang, Jinghua; Ochoa, Wendy F.; Sinkovits, Robert S. et al.
In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, No. 45, 11.11.2008, p. 17526-17531.

Research output: Contribution to journal › Article › peer-review

Tang, J, Ochoa, WF, Sinkovits, RS, Poulos, BT, Ghabrial, SA, Lightner, DV, Baker, TS & Nibert, ML 2008, 'Infectious myonecrosis virus has a totivirus-like, 120-subunit capsid, but with fiber complexes at the fivefold axes', Proceedings of the National Academy of Sciences of the United States of America, vol. 105, no. 45, pp. 17526-17531. https://doi.org/10.1073/pnas.0806724105

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abstract = "Infectious myonecrosis virus (IMNV) is an emerging pathogen of penaeid shrimp in global aquaculture. Tentatively assigned to family Totiviridae, it has a nonsegmented dsRNA genome of 7,560 bp and an isometric capsid of the 901-aa major capsid protein. We used electron cryomicroscopy and 3D image reconstruction to examine the IMNV virion at 8.0-{\AA} resolution. Results reveal a totivirus-like, 120-subunit T = 1 capsid, 450 {\AA} in diameter, but with fiber complexes protruding a further 80 {\AA} at the fivefold axes. These protrusions likely mediate roles in the extracellular transmission and pathogenesis of IMNV, capabilities not shared by most other totiviruses. The IMNV structure is also notable in that the genome is centrally organized in five or six concentric shells. Within each of these shells, the densities alternate between a dodecahedral frame that connects the threefold axes vs. concentration around the fivefold axes, implying certain regularities in the RNA packing scheme.",

keywords = "Electron cryomicroscopy, Nonenveloped virus, Penaeid shrimp, Totiviridae, dsRNA virus",

author = "Jinghua Tang and Ochoa, {Wendy F.} and Sinkovits, {Robert S.} and Poulos, {Bonnie T.} and Ghabrial, {Said A.} and Lightner, {Donald V.} and Baker, {Timothy S.} and Nibert, {Max L.}",

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AU - Poulos, Bonnie T.

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AU - Baker, Timothy S.

AU - Nibert, Max L.

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N2 - Infectious myonecrosis virus (IMNV) is an emerging pathogen of penaeid shrimp in global aquaculture. Tentatively assigned to family Totiviridae, it has a nonsegmented dsRNA genome of 7,560 bp and an isometric capsid of the 901-aa major capsid protein. We used electron cryomicroscopy and 3D image reconstruction to examine the IMNV virion at 8.0-Å resolution. Results reveal a totivirus-like, 120-subunit T = 1 capsid, 450 Å in diameter, but with fiber complexes protruding a further 80 Å at the fivefold axes. These protrusions likely mediate roles in the extracellular transmission and pathogenesis of IMNV, capabilities not shared by most other totiviruses. The IMNV structure is also notable in that the genome is centrally organized in five or six concentric shells. Within each of these shells, the densities alternate between a dodecahedral frame that connects the threefold axes vs. concentration around the fivefold axes, implying certain regularities in the RNA packing scheme.

AB - Infectious myonecrosis virus (IMNV) is an emerging pathogen of penaeid shrimp in global aquaculture. Tentatively assigned to family Totiviridae, it has a nonsegmented dsRNA genome of 7,560 bp and an isometric capsid of the 901-aa major capsid protein. We used electron cryomicroscopy and 3D image reconstruction to examine the IMNV virion at 8.0-Å resolution. Results reveal a totivirus-like, 120-subunit T = 1 capsid, 450 Å in diameter, but with fiber complexes protruding a further 80 Å at the fivefold axes. These protrusions likely mediate roles in the extracellular transmission and pathogenesis of IMNV, capabilities not shared by most other totiviruses. The IMNV structure is also notable in that the genome is centrally organized in five or six concentric shells. Within each of these shells, the densities alternate between a dodecahedral frame that connects the threefold axes vs. concentration around the fivefold axes, implying certain regularities in the RNA packing scheme.

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Infectious myonecrosis virus has a totivirus-like, 120-subunit capsid, but with fiber complexes at the fivefold axes

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