Abstract
The murine Ly-6A/E gene is transcriptionally induced in cells exposed to interferon α/β or γ (IFN-α/β or IFN-γ). Analysis of the 5′ flanking sequence using reporter plasmids that contain upstream elements of the Ly-6E gene has previously identified an ≈850-base-pair IFN-responsive region that lacked an IFN-α-stimulated response element (ISRE), the element present and required for an IFN-α response of a number of genes. Analysis by deletion and stable transfection of the IFN-responsive region of the Ly-6E promoter has defined an 80-base-pair region containing an IFN-γ activation site (GAS) but no ISRE that allows IFN-γ and IFN-α inducibility of the Ly-6E gene. As tested by specific antiserum, a 91-kDa protein known to be activated in IFN-α- or IFN-γ-treated cells binds to the GAS element from the Ly-6E promoter. The 91-kDa protein exists as an inactive cytoplasmic precursor and depends on tyrosine phosphorylation for its activation. Thus the same 91-kDa protein appears to act in the signal transduction pathways of both types of IFN for the Ly-6-A/E gene.
Original language | English (US) |
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Pages (from-to) | 6806-6810 |
Number of pages | 5 |
Journal | Proceedings of the National Academy of Sciences of the United States of America |
Volume | 90 |
Issue number | 14 |
State | Published - Jul 15 1993 |
Externally published | Yes |
Keywords
- Inducible transcription factor
- Interferon activation site
- Ly-6
- Tyrosine phosphorylation
ASJC Scopus subject areas
- General