Induction of the Ly-6A/E gene by interferon α/β and γ requires a DNA element to which a tyrosine-phosphorylated 91-kDa protein binds

Khuda Dad Khan, Ke Shuai, Glen Lindwall, Stephen E. Maher, J. E. Darnell, Alfred L.M. Bothwell

Research output: Contribution to journalArticlepeer-review

164 Scopus citations

Abstract

The murine Ly-6A/E gene is transcriptionally induced in cells exposed to interferon α/β or γ (IFN-α/β or IFN-γ). Analysis of the 5′ flanking sequence using reporter plasmids that contain upstream elements of the Ly-6E gene has previously identified an ≈850-base-pair IFN-responsive region that lacked an IFN-α-stimulated response element (ISRE), the element present and required for an IFN-α response of a number of genes. Analysis by deletion and stable transfection of the IFN-responsive region of the Ly-6E promoter has defined an 80-base-pair region containing an IFN-γ activation site (GAS) but no ISRE that allows IFN-γ and IFN-α inducibility of the Ly-6E gene. As tested by specific antiserum, a 91-kDa protein known to be activated in IFN-α- or IFN-γ-treated cells binds to the GAS element from the Ly-6E promoter. The 91-kDa protein exists as an inactive cytoplasmic precursor and depends on tyrosine phosphorylation for its activation. Thus the same 91-kDa protein appears to act in the signal transduction pathways of both types of IFN for the Ly-6-A/E gene.

Original languageEnglish (US)
Pages (from-to)6806-6810
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume90
Issue number14
StatePublished - Jul 15 1993
Externally publishedYes

Keywords

  • Inducible transcription factor
  • Interferon activation site
  • Ly-6
  • Tyrosine phosphorylation

ASJC Scopus subject areas

  • General

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