Induction of islet cytodifferentiation by fetal mesenchyme in adult pancreatic ductal epithelium

Ronald W. Dudek, Irvin E. Lawrence, Ronald S. Hill, Robert C. Johnson

Research output: Contribution to journalArticlepeer-review

96 Scopus citations


Recombinant tissue consisting of adult ductal epithelium isolated from pancreas and fetal mesenchyme was transplanted subcutaneously in the inguinal region of nude mice or epididymal fat pads of rats with a tissue chamber device for short-term (8-day) or long-term (6- to 12-wk) duration. We found that recombinant tissue underwent morphogenesis and cytodifferentiation, thereby forming islets that contained cells immunocytochemically positive for insulin and glucagon. Islet cytodifferentiation occurred in ∼20% of the recombinants. In recombinants that developed into islets, the tissue was always in close association with an extracellular matrix, nerves, and blood vessels. Controls consisting of mesenchyme alone or duct epithelium alone showed no evidence of morphogenesis of cytodifferentiation. Pancreatic rudiments were also implanted to serve as positive controls. This is the first demonstration of islet cytodifferentiation from adult duct epithelium.

Original languageEnglish (US)
Pages (from-to)1041-1048
Number of pages8
Issue number8
StatePublished - Aug 1991
Externally publishedYes

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism


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