Abstract
The human alloreactive T-cell clone A1 produces interleukin (IL)-4, IL-5, and granulocyte macrophage-colony stimulating factor (GM-CSF), but not IL-2 or interferon-γ (IFN-γ), as assessed by probing for mRNA transcripts, immunoassays, or bioassays. Supernatants from clone A1 induced IgE synthesis by normal human peripheral blood mononuclear cells. IL-4 was shown to be necessary and sufficient for the induction of IgE by clone A1 supernatants. In contrast, IgE induction by clone A1 supernatants and recombinant (r) IL-4 was inhibited by IFN-γ. This suggests that the absence of IFN-γ from the IL-4-containing A1 supernatants was important for their IgE-inducing ability. Supernatants from clone A1 could also specifically induce the growth of cord blood cell progenitors and their differentiation into eosinophils but not into basophils. rIL-5, but not rIL-4 or rGM-CSF, also induced eosinophil maturation. These data suggest that IL-5 secreted by clone A1 was responsible for its ability to induce eosinophil differentiation. The implications of the concomitant production of IL-4 and IL-5 by a single T-cell clone are discussed.
Original language | English (US) |
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Pages (from-to) | 437-446 |
Number of pages | 10 |
Journal | Journal of Clinical Immunology |
Volume | 8 |
Issue number | 6 |
DOIs | |
State | Published - Nov 1988 |
Externally published | Yes |
Keywords
- IL-5
- IgE synthesis
- Interleukin (IL)-4
- eosinophil differentiation
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology