Induction of CYP1A1 by β-naphthoflavone in IEC-18 rat intestinal epithelial cells and potentiation of induction by dibutyryl cAMP

Qing yu Zhang, Wenlei He, Deborah Dunbar, Laurence Kaminsky

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

We have examined the inducibility of CYP1A1 by β-naphthoflavone (BNF) in a rat intestinal epithelial cell line, IEC-18, and the associated interaction between cAMP and BNF. CYP1A1 was not constitutively expressed in IEC-18 cells. Upon treatment with BNF, CYP1A1 RNA, protein, and microsomal 7-ethoxyresorufin O-deethylase activity were detected. Treatment with dibutyryl cAMP resulted in a 2-fold increase in the extent of induction at both RNA and protein levels, with corresponding increases in CYP1A1 enzymatic activity. These results support the involvement of protein kinase A in Ah receptor-mediated induction of CYP1A1 and provide an in vitro model for further studies on the mechanisms underlying regional and cellular differences in the regulation of CYP1A1 gene expression in the small intestine.

Original languageEnglish (US)
Pages (from-to)623-626
Number of pages4
JournalBiochemical and Biophysical Research Communications
Volume233
Issue number3
DOIs
StatePublished - Apr 28 1997
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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