TY - JOUR
T1 - Induction of CYP1A1 by β-naphthoflavone in IEC-18 rat intestinal epithelial cells and potentiation of induction by dibutyryl cAMP
AU - Zhang, Qing yu
AU - He, Wenlei
AU - Dunbar, Deborah
AU - Kaminsky, Laurence
N1 - Funding Information:
This study was supported in part by Research Grant ES06256 from the National Institute of Environmental Health Sciences, National Institutes of Health. We would like to thank Dr. Xinxin Ding of the Wadsworth Center for helpful discussions.
PY - 1997/4/28
Y1 - 1997/4/28
N2 - We have examined the inducibility of CYP1A1 by β-naphthoflavone (BNF) in a rat intestinal epithelial cell line, IEC-18, and the associated interaction between cAMP and BNF. CYP1A1 was not constitutively expressed in IEC-18 cells. Upon treatment with BNF, CYP1A1 RNA, protein, and microsomal 7-ethoxyresorufin O-deethylase activity were detected. Treatment with dibutyryl cAMP resulted in a 2-fold increase in the extent of induction at both RNA and protein levels, with corresponding increases in CYP1A1 enzymatic activity. These results support the involvement of protein kinase A in Ah receptor-mediated induction of CYP1A1 and provide an in vitro model for further studies on the mechanisms underlying regional and cellular differences in the regulation of CYP1A1 gene expression in the small intestine.
AB - We have examined the inducibility of CYP1A1 by β-naphthoflavone (BNF) in a rat intestinal epithelial cell line, IEC-18, and the associated interaction between cAMP and BNF. CYP1A1 was not constitutively expressed in IEC-18 cells. Upon treatment with BNF, CYP1A1 RNA, protein, and microsomal 7-ethoxyresorufin O-deethylase activity were detected. Treatment with dibutyryl cAMP resulted in a 2-fold increase in the extent of induction at both RNA and protein levels, with corresponding increases in CYP1A1 enzymatic activity. These results support the involvement of protein kinase A in Ah receptor-mediated induction of CYP1A1 and provide an in vitro model for further studies on the mechanisms underlying regional and cellular differences in the regulation of CYP1A1 gene expression in the small intestine.
UR - https://www.scopus.com/pages/publications/0031589143
UR - https://www.scopus.com/inward/citedby.url?scp=0031589143&partnerID=8YFLogxK
U2 - 10.1006/bbrc.1997.6508
DO - 10.1006/bbrc.1997.6508
M3 - Article
C2 - 9168901
AN - SCOPUS:0031589143
SN - 0006-291X
VL - 233
SP - 623
EP - 626
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -