TY - JOUR
T1 - Induction of activation antigens on human natural killer cells mediated through the Fc-γ receptors
AU - Harris, D. T.
AU - Travis, W. W.
AU - Koren, H. S.
PY - 1989
Y1 - 1989
N2 - NK cells, defined here as lymphocytes bearing the CD16 Ag found on the NK cell Fc-γ receptor (FcR), are known to enter a proliferative and activated state in response to stimulation with IL-2 as assessed by clonal expansion, short-term DNA synthesis, and de novo expression of lymphocyte-associated activation Ag. We have found that the Fcr of NK cells acts as a signaling pathway through which IL-2-dependent activation may be greatly enhanced, allowing for more rapid induction of activation Ag and recruitment of an increased percentage of cells expressing surface markers of cellular activation. FcR-interactive agents, such as solid phase immobilized immune complexes or cross-linked CD16-specific mAb, work synergistically with rIL-2 to elicit a rapid expression of IL2R and transferrin receptors on greater than 50% of purified NK cells as early as day 3 of culture. IL-2 or FcR-interactive stimuli alone were weak or ineffective stimulators by comparison. In contrast to the induction of de novo activation Ag, DNA synthesis was elicited by IL-2-alone, but was not substantially or consistently enhanced by the subsequent addition of FcR-interactive stimuli.
AB - NK cells, defined here as lymphocytes bearing the CD16 Ag found on the NK cell Fc-γ receptor (FcR), are known to enter a proliferative and activated state in response to stimulation with IL-2 as assessed by clonal expansion, short-term DNA synthesis, and de novo expression of lymphocyte-associated activation Ag. We have found that the Fcr of NK cells acts as a signaling pathway through which IL-2-dependent activation may be greatly enhanced, allowing for more rapid induction of activation Ag and recruitment of an increased percentage of cells expressing surface markers of cellular activation. FcR-interactive agents, such as solid phase immobilized immune complexes or cross-linked CD16-specific mAb, work synergistically with rIL-2 to elicit a rapid expression of IL2R and transferrin receptors on greater than 50% of purified NK cells as early as day 3 of culture. IL-2 or FcR-interactive stimuli alone were weak or ineffective stimulators by comparison. In contrast to the induction of de novo activation Ag, DNA synthesis was elicited by IL-2-alone, but was not substantially or consistently enhanced by the subsequent addition of FcR-interactive stimuli.
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M3 - Article
C2 - 2528593
AN - SCOPUS:0024343183
VL - 143
SP - 2401
EP - 2406
JO - Journal of Immunology
JF - Journal of Immunology
SN - 0022-1767
IS - 7
ER -