Induction and potentiation of lethal and genetic effects of ultraviolet light by tobacco smoke condensates in yeast

Mohammed A. Hannan; R. Scott Estes; Laurence H. Hurley

doi:10.1016/0013-9351(80)90012-2

Induction and potentiation of lethal and genetic effects of ultraviolet light by tobacco smoke condensates in yeast

Mohammed A. Hannan, R. Scott Estes, Laurence H. Hurley

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Tobacco smoke condensates (TSC) were tested for DNA repair inhibition in both repair proficient and different classes of repair deficient strains of Saccharomyces cerevisiae. TSC was also tested for induction and potentiation of mutations and mitotic gene conversion in unirradiated and uv-irradiated yeast cells. TSC was found to sensitize all the strains of yeast to uv-inactivation indicating that it acts in a nonspecific manner and does not specifically inhibit a particular repair pathway. Genetic studies showed that TSC, without exogenous metabolic activation, failed to produce mutations while it induced mitotic gene conversion in the diploid strain. At specific concentrations, TSC potentiated both mutagenic and gene convertogenic effects uv-light while at higher concentrations of TSC a reduction of mutations was observed. The results are discussed as they relate to carcinogenesis and cocarcinogenesis/tumor promotion.

Original language	English (US)
Pages (from-to)	97-107
Number of pages	11
Journal	Environmental Research
Volume	21
Issue number	1
DOIs	https://doi.org/10.1016/0013-9351(80)90012-2
State	Published - Feb 1980
Externally published	Yes

ASJC Scopus subject areas

Biochemistry
General Environmental Science

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10.1016/0013-9351(80)90012-2

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title = "Induction and potentiation of lethal and genetic effects of ultraviolet light by tobacco smoke condensates in yeast",

abstract = "Tobacco smoke condensates (TSC) were tested for DNA repair inhibition in both repair proficient and different classes of repair deficient strains of Saccharomyces cerevisiae. TSC was also tested for induction and potentiation of mutations and mitotic gene conversion in unirradiated and uv-irradiated yeast cells. TSC was found to sensitize all the strains of yeast to uv-inactivation indicating that it acts in a nonspecific manner and does not specifically inhibit a particular repair pathway. Genetic studies showed that TSC, without exogenous metabolic activation, failed to produce mutations while it induced mitotic gene conversion in the diploid strain. At specific concentrations, TSC potentiated both mutagenic and gene convertogenic effects uv-light while at higher concentrations of TSC a reduction of mutations was observed. The results are discussed as they relate to carcinogenesis and cocarcinogenesis/tumor promotion.",

author = "Hannan, {Mohammed A.} and Estes, {R. Scott} and Hurley, {Laurence H.}",

note = "Funding Information: The studies were supported by a grant from the University of Kentucky Tobacco and Health Institute.",

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journal = "Environmental Research",

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T1 - Induction and potentiation of lethal and genetic effects of ultraviolet light by tobacco smoke condensates in yeast

AU - Hannan, Mohammed A.

AU - Estes, R. Scott

AU - Hurley, Laurence H.

N1 - Funding Information: The studies were supported by a grant from the University of Kentucky Tobacco and Health Institute.

PY - 1980/2

Y1 - 1980/2

N2 - Tobacco smoke condensates (TSC) were tested for DNA repair inhibition in both repair proficient and different classes of repair deficient strains of Saccharomyces cerevisiae. TSC was also tested for induction and potentiation of mutations and mitotic gene conversion in unirradiated and uv-irradiated yeast cells. TSC was found to sensitize all the strains of yeast to uv-inactivation indicating that it acts in a nonspecific manner and does not specifically inhibit a particular repair pathway. Genetic studies showed that TSC, without exogenous metabolic activation, failed to produce mutations while it induced mitotic gene conversion in the diploid strain. At specific concentrations, TSC potentiated both mutagenic and gene convertogenic effects uv-light while at higher concentrations of TSC a reduction of mutations was observed. The results are discussed as they relate to carcinogenesis and cocarcinogenesis/tumor promotion.

AB - Tobacco smoke condensates (TSC) were tested for DNA repair inhibition in both repair proficient and different classes of repair deficient strains of Saccharomyces cerevisiae. TSC was also tested for induction and potentiation of mutations and mitotic gene conversion in unirradiated and uv-irradiated yeast cells. TSC was found to sensitize all the strains of yeast to uv-inactivation indicating that it acts in a nonspecific manner and does not specifically inhibit a particular repair pathway. Genetic studies showed that TSC, without exogenous metabolic activation, failed to produce mutations while it induced mitotic gene conversion in the diploid strain. At specific concentrations, TSC potentiated both mutagenic and gene convertogenic effects uv-light while at higher concentrations of TSC a reduction of mutations was observed. The results are discussed as they relate to carcinogenesis and cocarcinogenesis/tumor promotion.

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Induction and potentiation of lethal and genetic effects of ultraviolet light by tobacco smoke condensates in yeast

Abstract

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