Increased cardiac myosin super-relaxation as an energy saving mechanism in hibernating grizzly bears

Robbert J. Van der Pijl; Weikang Ma; Christopher T.A. Lewis; Line Haar; Amalie Buhl; Gerrie P. Farman; Marcus Rhodehamel; Vivek P. Jani; O. Lynne Nelson; Chengxin Zhang; Henk Granzier; Julien Ochala

doi:10.1016/j.molmet.2024.102084

Increased cardiac myosin super-relaxation as an energy saving mechanism in hibernating grizzly bears

Robbert J. Van der Pijl, Weikang Ma, Christopher T.A. Lewis, Line Haar, Amalie Buhl, Gerrie P. Farman, Marcus Rhodehamel, Vivek P. Jani, O. Lynne Nelson, Chengxin Zhang, Henk Granzier, Julien Ochala

Research output: Contribution to journal › Article › peer-review

Abstract

Aim: The aim of the present study was to define whether cardiac myosin contributes to energy conservation in the heart of hibernating mammals. Methods: Thin cardiac strips were isolated from the left ventricles of active and hibernating grizzly bears; and subjected to loaded Mant-ATP chase assays, X-ray diffraction and proteomics. Main findings: Hibernating grizzly bears displayed an unusually high proportion of ATP-conserving super-relaxed cardiac myosin molecules that are likely due to altered levels of phosphorylation and rod region stability. Conclusions: Cardiac myosin depresses the heart's energetic demand during hibernation by modulating its function.

Original language	English (US)
Article number	102084
Journal	Molecular Metabolism
Volume	92
DOIs	https://doi.org/10.1016/j.molmet.2024.102084
State	Published - Feb 2025
Externally published	Yes

Keywords

Heart
Hibernation
Metabolism
Myosin

ASJC Scopus subject areas

Molecular Biology
Cell Biology

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10.1016/j.molmet.2024.102084

Cite this

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title = "Increased cardiac myosin super-relaxation as an energy saving mechanism in hibernating grizzly bears",

abstract = "Aim: The aim of the present study was to define whether cardiac myosin contributes to energy conservation in the heart of hibernating mammals. Methods: Thin cardiac strips were isolated from the left ventricles of active and hibernating grizzly bears; and subjected to loaded Mant-ATP chase assays, X-ray diffraction and proteomics. Main findings: Hibernating grizzly bears displayed an unusually high proportion of ATP-conserving super-relaxed cardiac myosin molecules that are likely due to altered levels of phosphorylation and rod region stability. Conclusions: Cardiac myosin depresses the heart's energetic demand during hibernation by modulating its function.",

keywords = "Heart, Hibernation, Metabolism, Myosin",

author = "{Van der Pijl}, {Robbert J.} and Weikang Ma and Lewis, {Christopher T.A.} and Line Haar and Amalie Buhl and Farman, {Gerrie P.} and Marcus Rhodehamel and Jani, {Vivek P.} and Nelson, {O. Lynne} and Chengxin Zhang and Henk Granzier and Julien Ochala",

note = "Publisher Copyright: {\textcopyright} 2024 The Author(s)",

year = "2025",

month = feb,

doi = "10.1016/j.molmet.2024.102084",

language = "English (US)",

volume = "92",

journal = "Molecular Metabolism",

issn = "2212-8778",

publisher = "Elsevier GmbH",

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T1 - Increased cardiac myosin super-relaxation as an energy saving mechanism in hibernating grizzly bears

AU - Van der Pijl, Robbert J.

AU - Ma, Weikang

AU - Lewis, Christopher T.A.

AU - Haar, Line

AU - Buhl, Amalie

AU - Farman, Gerrie P.

AU - Rhodehamel, Marcus

AU - Jani, Vivek P.

AU - Nelson, O. Lynne

AU - Zhang, Chengxin

AU - Granzier, Henk

AU - Ochala, Julien

PY - 2025/2

Y1 - 2025/2

N2 - Aim: The aim of the present study was to define whether cardiac myosin contributes to energy conservation in the heart of hibernating mammals. Methods: Thin cardiac strips were isolated from the left ventricles of active and hibernating grizzly bears; and subjected to loaded Mant-ATP chase assays, X-ray diffraction and proteomics. Main findings: Hibernating grizzly bears displayed an unusually high proportion of ATP-conserving super-relaxed cardiac myosin molecules that are likely due to altered levels of phosphorylation and rod region stability. Conclusions: Cardiac myosin depresses the heart's energetic demand during hibernation by modulating its function.

AB - Aim: The aim of the present study was to define whether cardiac myosin contributes to energy conservation in the heart of hibernating mammals. Methods: Thin cardiac strips were isolated from the left ventricles of active and hibernating grizzly bears; and subjected to loaded Mant-ATP chase assays, X-ray diffraction and proteomics. Main findings: Hibernating grizzly bears displayed an unusually high proportion of ATP-conserving super-relaxed cardiac myosin molecules that are likely due to altered levels of phosphorylation and rod region stability. Conclusions: Cardiac myosin depresses the heart's energetic demand during hibernation by modulating its function.

KW - Heart

KW - Hibernation

KW - Metabolism

KW - Myosin

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Increased cardiac myosin super-relaxation as an energy saving mechanism in hibernating grizzly bears

Abstract

Keywords

ASJC Scopus subject areas

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