Abstract
The effects of ethanol exposures on calcium/calmodulin-dependent protein kinase activity as well as its influence on glutamate uptake were determined in astrocytes prepared from neonatal rat cerebral cortex. Acute 15-min exposure to 100 mM ethanol had no effect on Ca2+/CaM-dependent protein kinase activity. However, chronic exposure to 100 mM ethanol for 4 days elicited a significant increase in the activity of this enzyme with no parallel increase in its expression. 2+ Ca/CaM-independent kinase activity was less than 1% of the Ca2+/CaM-dependent kinase activity and was unaffected by any of the ethanol exposures. Exposure to 100 mM ethanol for four days also resulted in a significant increase in Na+dependent [3H] glutamate uptake which was reversed when ethanol-exposed astrocytes were co- incubated with KN-93, a specific inhibitor of Ca2+/CaM kinase. These results suggest that the effects of ethanol on glutamate transport may be mediated in part, by the level of Ca2+/CaM kinase activity.
Original language | English (US) |
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Pages (from-to) | 145-148 |
Number of pages | 4 |
Journal | Neuroscience Letters |
Volume | 269 |
Issue number | 3 |
DOIs | |
State | Published - Jul 16 1999 |
Keywords
- Astrocytes
- Ca/CaM kinase activity
- Chronic ethanol
- Glutamate transport
ASJC Scopus subject areas
- Neuroscience(all)