Increased calcium/calmodulin protein kinase activity in astrocytes chronically exposed to ethanol: Influences on glutamate transport

Thomas L. Smith, Edita Navratilova

Research output: Contribution to journalArticlepeer-review

29 Scopus citations

Abstract

The effects of ethanol exposures on calcium/calmodulin-dependent protein kinase activity as well as its influence on glutamate uptake were determined in astrocytes prepared from neonatal rat cerebral cortex. Acute 15-min exposure to 100 mM ethanol had no effect on Ca2+/CaM-dependent protein kinase activity. However, chronic exposure to 100 mM ethanol for 4 days elicited a significant increase in the activity of this enzyme with no parallel increase in its expression. 2+ Ca/CaM-independent kinase activity was less than 1% of the Ca2+/CaM-dependent kinase activity and was unaffected by any of the ethanol exposures. Exposure to 100 mM ethanol for four days also resulted in a significant increase in Na+dependent [3H] glutamate uptake which was reversed when ethanol-exposed astrocytes were co- incubated with KN-93, a specific inhibitor of Ca2+/CaM kinase. These results suggest that the effects of ethanol on glutamate transport may be mediated in part, by the level of Ca2+/CaM kinase activity.

Original languageEnglish (US)
Pages (from-to)145-148
Number of pages4
JournalNeuroscience Letters
Volume269
Issue number3
DOIs
StatePublished - Jul 16 1999

Keywords

  • Astrocytes
  • Ca/CaM kinase activity
  • Chronic ethanol
  • Glutamate transport

ASJC Scopus subject areas

  • Neuroscience(all)

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