TY - JOUR
T1 - Inactivation of MS2 coliphage by UV and hydrogen peroxide
T2 - Comparison by cultural and molecular methodologies
AU - Sherchan, Samendra P.
AU - Snyder, Shane A.
AU - Gerba, Charles P.
AU - Pepper, Ian L.
N1 - Funding Information:
This work was supported by the University of Arizona National Science Foundation Water and Environmental Technology Center. The comments and views detailed herein may not necessarily reflect the views of the funding agency.
PY - 2014/3/21
Y1 - 2014/3/21
N2 - The use of advanced oxidation processes (AOP) are expected to increase for removal of emerging contaminants and pathogens from drinking water. In this study, the performance of a small community ultraviolet light reactor in combination with hydrogen peroxide (H2O2) for MS2 coliphage inactivation with two different flow rate conditions of 1 gal/min (gpm) and 2 gpm was evaluated. FollowingUVradiation,MS2showed a reduction of 5.3-5.8 log10 when quantified with cultural plaque counts,whereas corresponding quantitative polymerase chain reaction (qPCR) data showed only a 1.7-2.8 log10 reduction in viral RNA copy number. When H2O2 was added at either 2.5 or 5 ppm with UV at both flow rate conditions, enhanced MS2 inactivation occurred with a more than 7 log10 reduction observed via plaque counts, indicating that all added MS2 had been inactivated, since no plaques were formed after incubation at 37°C for 24 h. In contrast, qPCR only showed a corresponding 3-4 log10 reduction in viral RNA copy number. This research also sheds light on the inactivation of MS2 with ultraviolet light and in the presence of hydroxyl radicals and provides a practical use of qPCR to detect MS2 concentration following advanced oxidation relative to traditional plaque methodology; however qPCR detection overestimates the true number of infective virus.
AB - The use of advanced oxidation processes (AOP) are expected to increase for removal of emerging contaminants and pathogens from drinking water. In this study, the performance of a small community ultraviolet light reactor in combination with hydrogen peroxide (H2O2) for MS2 coliphage inactivation with two different flow rate conditions of 1 gal/min (gpm) and 2 gpm was evaluated. FollowingUVradiation,MS2showed a reduction of 5.3-5.8 log10 when quantified with cultural plaque counts,whereas corresponding quantitative polymerase chain reaction (qPCR) data showed only a 1.7-2.8 log10 reduction in viral RNA copy number. When H2O2 was added at either 2.5 or 5 ppm with UV at both flow rate conditions, enhanced MS2 inactivation occurred with a more than 7 log10 reduction observed via plaque counts, indicating that all added MS2 had been inactivated, since no plaques were formed after incubation at 37°C for 24 h. In contrast, qPCR only showed a corresponding 3-4 log10 reduction in viral RNA copy number. This research also sheds light on the inactivation of MS2 with ultraviolet light and in the presence of hydroxyl radicals and provides a practical use of qPCR to detect MS2 concentration following advanced oxidation relative to traditional plaque methodology; however qPCR detection overestimates the true number of infective virus.
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U2 - 10.1080/10934529.2014.854607
DO - 10.1080/10934529.2014.854607
M3 - Article
C2 - 24345237
AN - SCOPUS:84890810899
SN - 1093-4529
VL - 49
SP - 397
EP - 403
JO - Journal of Environmental Science and Health - Part A Toxic/Hazardous Substances and Environmental Engineering
JF - Journal of Environmental Science and Health - Part A Toxic/Hazardous Substances and Environmental Engineering
IS - 4
ER -