In vivo time-serial multi-modality optical imaging in a mouse model of ovarian tumorigenesis

Jennifer M. Watson, Samuel L. Marion, Photini F. Rice, David L. Bentley, David G. Besselsen, Urs Utzinger, Patricia B. Hoyer, Jennifer K. Barton

Research output: Contribution to journalArticlepeer-review

15 Scopus citations


Identification of the early microscopic changes associated with ovarian cancer may lead to development of a diagnostic test for high-risk women. In this study we use optical coherence tomography (OCT) and multiphoton microscopy (MPM) (collecting both two photon excited fluorescence [TPEF] and second harmonic generation [SH G]) to image mouse ovaries in vivo at multiple time points. We demonstrate the feasibility of imaging mouse ovaries in vivo during a longterm survival study and identify microscopic changes associated with early tumor development. These changes include alterations in tissue microstructure, as seen by OCT, alterations in cellular fluorescence and morphology, as seen by TPEF, and remodeling of collagen structure, as seen by SH G. These results suggest that a combined OCT-MPM system may be useful for early detection of ovarian cancer.

Original languageEnglish (US)
Pages (from-to)42-60
Number of pages19
JournalCancer Biology and Therapy
Issue number1
StatePublished - Jan 2014


  • Autofluorescence
  • Cancer
  • Multiphoton
  • Non-linear microscopy
  • Optical biopsy
  • Optical coherence tomography
  • Two-photon

ASJC Scopus subject areas

  • Molecular Medicine
  • Oncology
  • Pharmacology
  • Cancer Research


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