In vivo three-dimensional optical coherence tomography and multiphoton microscopy in a mouse model of ovarian neoplasia

Jennifer M. Watson, Samuel L. Marion, Photini Faith Rice, David L. Bentley, David Besselsen, Urs Utzinger, Patricia B Hoyer, Jennifer K. Barton

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Our goal is to use optical coherence tomography (OCT) and multiphoton microscopy (MPM) to detect early tumor development in a mouse model of ovarian neoplasia. We hope to use information regarding early tumor development to create a diagnostic test for high-risk patients. In this study we collect in vivo images using OCT, second harmonic generation and two-photon excited fluorescence from non-vinylcyclohexene diepoxide (VCD)-dosed and VCD-dosed mice. VCD causes follicular apoptosis (simulating menopause) and leads to tumor development. Using OCT and MPM we visualized the ovarian microstructure and were able to see differences between non-VCD-dosed and VCD-dosed animals. This leads us to believe that OCT and MPM may be useful for detecting changes due to early tumor development.

Original languageEnglish (US)
Title of host publicationOptical Biopsy XI
DOIs
StatePublished - 2013
EventConference on Optical Biopsy XI - San Francisco, CA, United States
Duration: Feb 5 2013Feb 6 2013

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume8577
ISSN (Print)1605-7422

Other

OtherConference on Optical Biopsy XI
Country/TerritoryUnited States
CitySan Francisco, CA
Period2/5/132/6/13

Keywords

  • fluorescence microscopy
  • imaging
  • ovarian cancer
  • post-menopausal mouse model
  • second harmonic generation
  • two-photon excited fluorescence
  • vinylcyclohexene diepoxide

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

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