In vivo imaging of embryonic stem cells reveals patterns of survival and immune rejection following transplantation

Rutger Jan Swijnenburg, Sonja Schrepfer, Feng Cao, Jeremy I. Pearl, Xiaoyan Xie, Andrew J. Connolly, Robert C. Robbins, Joseph C. Wu

Research output: Contribution to journalArticlepeer-review

96 Scopus citations

Abstract

Embryonic stem cell (ESC)-based transplantation is considered a promising novel therapy for a variety of diseases. This is bolstered by the suggested immune-privileged properties of ESCs. In this study, we used in vivo bioluminescent imaging (BLI) to non-invasively track the fate of transplanted murine ESCs (mESCs), which are stably transduced with a double fusion reporter gene consisting of firefly luciferase (FLuc) and enhanced green fluorescent protein (eGFP). Following syngeneic intramuscular transplantation of 1 × 106 mESCs, the cells survived and differentiated into teratomas. In contrast, allogeneic mESC transplants were infiltrated by a variety of inflammatory cells, leading to rejection within 28 days. Acceleration of rejection was observed when mESCs were allotransplanted following prior sensitization of the host. Finally, we demonstrate that the mESC derivatives were more rapidly rejected compared to undifferentiated mESCs. These data show that mESCs do not retain immune-privileged properties in vivo and are subject to immunological rejection as assessed by novel molecular imaging approaches.

Original languageEnglish (US)
Pages (from-to)1023-1029
Number of pages7
JournalStem Cells and Development
Volume17
Issue number6
DOIs
StatePublished - Dec 2008
Externally publishedYes

ASJC Scopus subject areas

  • Hematology
  • Developmental Biology
  • Cell Biology

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