In vivo evidence of inflammasome activation during spontaneous labor at term

Bogdan Panaitescu, Roberto Romero, Nardhy Gomez-Lopez, Yi Xu, Yaozhu Leng, Eli Maymon, Percy Pacora, Offer Erez, Lami Yeo, Sonia S. Hassan, Chaur Dong Hsu

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

Objective: Upon inflammasome activation, the adaptor protein of the inflammasome ASC (apoptosis-associated speck-like protein containing a CARD) forms intracellular specks, which can be released into the extracellular space. The objectives of this study were to investigate whether (1) extracellular ASC is present in the amniotic fluid of women who delivered at term; (2) amniotic fluid ASC concentrations are greater in women who underwent spontaneous labor at term than in those who delivered at term in the absence of labor; and (3) amniotic epithelial and mesenchymal cells can form intracellular ASC specks in vitro. Methods: This retrospective cross-sectional study included amniotic fluid samples from 41 women who delivered at term in the absence of labor (n = 24) or underwent spontaneous labor at term (n = 17). Amniotic epithelial and mesenchymal cells were also isolated from the chorioamniotic membranes obtained from a separate group of women who delivered at term (n = 3), in which ASC speck formation was assessed by confocal microscopy. Monocytes from healthy individuals were used as positive controls for ASC speck formation (n = 3). Results: (1) The adaptor protein of the inflammasome ASC is detectable in the amniotic fluid of women who delivered at term; (2) amniotic fluid ASC concentration was higher in women who underwent spontaneous labor at term than in those who delivered at term without labor; and (3) amniotic epithelial and mesenchymal cells are capable of forming ASC specks and/or filaments in vitro. Conclusion: Amniotic fluid ASC concentrations are increased in women who undergo spontaneous labor at term. Amniotic epithelial and mesenchymal cells are capable of forming ASC specks, suggesting that these cells are a source of extracellular ASC in the amniotic fluid. These findings provide in vivo evidence that there is inflammasome activation in the amniotic cavity during the physiological process of labor at term.

Original languageEnglish (US)
Pages (from-to)1978-1991
Number of pages14
JournalJournal of Maternal-Fetal and Neonatal Medicine
Volume32
Issue number12
DOIs
StatePublished - Jun 18 2019
Externally publishedYes

Keywords

  • ASC speck
  • Amnion
  • PYD and CARD Domain-Containing Protein (Pycard)
  • amniotic fluid
  • apoptosis-associated speck-like protein containing a CARD (ASC)
  • caspase-1
  • chorioamniotic membranes
  • chorion
  • cytokine
  • interleukin-18
  • interleukin-1beta
  • intra-amniotic inflammation
  • parturition
  • pattern recognition receptor (PRR)
  • pregnancy
  • sterile inflammation

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health
  • Obstetrics and Gynecology

Fingerprint

Dive into the research topics of 'In vivo evidence of inflammasome activation during spontaneous labor at term'. Together they form a unique fingerprint.

Cite this