TY - JOUR
T1 - In vivo and in vitro analysis of Bordetella pertussis catalase and Fe-superoxide dismutase mutants
AU - Khelef, Nadia
AU - DeShazer, David
AU - Friedman, Richard L.
AU - Guiso, Nicole
N1 - Funding Information:
We are gratefult o SabineP oiret and Christian Weber for excellentt echnical assistanceS. pecial thanksg o to James Moulder and Gwen Wood for their critical review of this paper. This work was supportedb y the Institut PasteurF oundationa nd the LaboratoiresV irbac.
PY - 1996/9/1
Y1 - 1996/9/1
N2 - Bordetella pertussis produces a catalase and a Fe-superoxide dismutase. The importance of these enzymes in virulence was investigated, in vitro as well as in vivo, by using mutants deficient in their production. The catalase-deficient mutant survived within polymorphonuclear leukocytes, killed 5774A.1 macrophages through apoptosis, and behaved as the parental strain in a murine respiratory infection model. These results suggest no direct role for catalase in B. pertussis virulence. The absence of expression of Fe-superoxide dismutase had profound effects on the bacterium including a reduced ability to express adenylate cyclase-hemolysin and pertactin, two factors important for B. pertussis pathogenesis. The Fe-superoxide dismutase-deficient mutant also had decreased abilities to colonize and persist in the murine respiratory infection model.
AB - Bordetella pertussis produces a catalase and a Fe-superoxide dismutase. The importance of these enzymes in virulence was investigated, in vitro as well as in vivo, by using mutants deficient in their production. The catalase-deficient mutant survived within polymorphonuclear leukocytes, killed 5774A.1 macrophages through apoptosis, and behaved as the parental strain in a murine respiratory infection model. These results suggest no direct role for catalase in B. pertussis virulence. The absence of expression of Fe-superoxide dismutase had profound effects on the bacterium including a reduced ability to express adenylate cyclase-hemolysin and pertactin, two factors important for B. pertussis pathogenesis. The Fe-superoxide dismutase-deficient mutant also had decreased abilities to colonize and persist in the murine respiratory infection model.
KW - Bordetella pertussis
KW - Catalase
KW - Superoxide dismutase
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U2 - 10.1016/0378-1097(96)00283-2
DO - 10.1016/0378-1097(96)00283-2
M3 - Article
C2 - 8810507
AN - SCOPUS:0030245610
SN - 0378-1097
VL - 142
SP - 231
EP - 235
JO - FEMS Microbiology Letters
JF - FEMS Microbiology Letters
IS - 2-3
ER -