In vitro RNA synthesis with SP6 RNA polymerase

P. A. Krieg; D. A. Melton

In vitro RNA synthesis with SP6 RNA polymerase

P. A. Krieg
, D. A. Melton

Cellular and Molecular Medicine

Research output: Contribution to journal › Article › peer-review

673 Scopus citations

Abstract

In this chapter we describe the use of in vitro transcription systems for the synthesis of RNAs for use as substrates and hybridization probes. We discuss advantages of the technique and several associated problems. Because our experience is primarily with SP6 RNA polymerase we will use this system as an example throughout, but we believe the observations are general and apply to any of the phage polymerase and promoter systems, including T3 and T7.

Original language	English (US)
Pages (from-to)	397-415
Number of pages	19
Journal	Methods in Enzymology
Volume	155
State	Published - 1987

ASJC Scopus subject areas

Biochemistry
Molecular Biology

Cite this

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title = "In vitro RNA synthesis with SP6 RNA polymerase",

abstract = "In this chapter we describe the use of in vitro transcription systems for the synthesis of RNAs for use as substrates and hybridization probes. We discuss advantages of the technique and several associated problems. Because our experience is primarily with SP6 RNA polymerase we will use this system as an example throughout, but we believe the observations are general and apply to any of the phage polymerase and promoter systems, including T3 and T7.",

author = "Krieg, \{P. A.\} and Melton, \{D. A.\}",

note = "Funding Information: We thank Richard Harvey, Chris Kintner, Heather O'Keefe, and Dan Weeks for comments on the manuscript. This work was supported by grants from the NIH and the Chicago Community Trust/Searle Scholars Program.",

year = "1987",

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journal = "Methods in Enzymology",

issn = "0076-6879",

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AU - Krieg, P. A.

AU - Melton, D. A.

N1 - Funding Information: We thank Richard Harvey, Chris Kintner, Heather O'Keefe, and Dan Weeks for comments on the manuscript. This work was supported by grants from the NIH and the Chicago Community Trust/Searle Scholars Program.

PY - 1987

Y1 - 1987

N2 - In this chapter we describe the use of in vitro transcription systems for the synthesis of RNAs for use as substrates and hybridization probes. We discuss advantages of the technique and several associated problems. Because our experience is primarily with SP6 RNA polymerase we will use this system as an example throughout, but we believe the observations are general and apply to any of the phage polymerase and promoter systems, including T3 and T7.

AB - In this chapter we describe the use of in vitro transcription systems for the synthesis of RNAs for use as substrates and hybridization probes. We discuss advantages of the technique and several associated problems. Because our experience is primarily with SP6 RNA polymerase we will use this system as an example throughout, but we believe the observations are general and apply to any of the phage polymerase and promoter systems, including T3 and T7.

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M3 - Article

C2 - 2828872

AN - SCOPUS:0023623129

SN - 0076-6879

VL - 155

SP - 397

EP - 415

JO - Methods in Enzymology

JF - Methods in Enzymology

ER -

In vitro RNA synthesis with SP6 RNA polymerase

Abstract

ASJC Scopus subject areas

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