In vitro conversion of proinsulin to insulin by cathepsin B in isolated islets and its inhibition by cathepsin B antibodies

Renu Bansal; Nafees Ahmad; Jalil R. Kidwai

doi:10.1007/BF02581326

In vitro conversion of proinsulin to insulin by cathepsin B in isolated islets and its inhibition by cathepsin B antibodies

Renu Bansal, Nafees Ahmad, Jalil R. Kidwai

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Purified bovine cathepsin B, when incubated with isolated rat islets of Langerhans, completely converts proinsulin to insulin as demonstrated by the incorporation of¹⁴C-leucine into islet proteins, releasing lysine as the only basic amino acid. Cathepsin B antibodies raised in rabbit inhibited the above conversion.

Original language	English (US)
Pages (from-to)	255-266
Number of pages	12
Journal	Acta Diabetologica Latina
Volume	17
Issue number	3-4
DOIs	https://doi.org/10.1007/BF02581326
State	Published - Jul 1980
Externally published	Yes

Keywords

Cathepsin B
Cathepsin B antibodies
Islets of Langerhans
Proinsulin-insulin

ASJC Scopus subject areas

Internal Medicine
Endocrinology, Diabetes and Metabolism
Endocrinology

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10.1007/BF02581326

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title = "In vitro conversion of proinsulin to insulin by cathepsin B in isolated islets and its inhibition by cathepsin B antibodies",

abstract = "Purified bovine cathepsin B, when incubated with isolated rat islets of Langerhans, completely converts proinsulin to insulin as demonstrated by the incorporation of14C-leucine into islet proteins, releasing lysine as the only basic amino acid. Cathepsin B antibodies raised in rabbit inhibited the above conversion.",

keywords = "Cathepsin B, Cathepsin B antibodies, Islets of Langerhans, Proinsulin-insulin",

author = "Renu Bansal and Nafees Ahmad and Kidwai, {Jalil R.}",

year = "1980",

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AU - Bansal, Renu

AU - Ahmad, Nafees

AU - Kidwai, Jalil R.

PY - 1980/7

Y1 - 1980/7

N2 - Purified bovine cathepsin B, when incubated with isolated rat islets of Langerhans, completely converts proinsulin to insulin as demonstrated by the incorporation of14C-leucine into islet proteins, releasing lysine as the only basic amino acid. Cathepsin B antibodies raised in rabbit inhibited the above conversion.

AB - Purified bovine cathepsin B, when incubated with isolated rat islets of Langerhans, completely converts proinsulin to insulin as demonstrated by the incorporation of14C-leucine into islet proteins, releasing lysine as the only basic amino acid. Cathepsin B antibodies raised in rabbit inhibited the above conversion.

KW - Cathepsin B

KW - Cathepsin B antibodies

KW - Islets of Langerhans

KW - Proinsulin-insulin

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In vitro conversion of proinsulin to insulin by cathepsin B in isolated islets and its inhibition by cathepsin B antibodies

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

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