In Vitro Assessment of Cellular Immunity in Human Coccidioidomycosis: Relationship between Dermal Hypersensitivity, Lymphocyte Transformation, and Lymphokine Production by Peripheral Blood Mononuclear Cells from Healthy Adults

Neil M. Ampel; George C. Bejarano; Steven D. Salas; John N. Galgiani

doi:10.1093/infdis/165.4.710

In Vitro Assessment of Cellular Immunity in Human Coccidioidomycosis: Relationship between Dermal Hypersensitivity, Lymphocyte Transformation, and Lymphokine Production by Peripheral Blood Mononuclear Cells from Healthy Adults

Neil M. Ampel, George C. Bejarano, Steven D. Salas, John N. Galgiani

Research output: Contribution to journal › Article › peer-review

31 Scopus citations

Abstract

Dermal hypersensitivity in coccidioidomycosis was compared with three simultaneous measures of in vitro cellular immunity using 35 healthy donors living in an area endemic for coccidioidomycosis. Twenty donors had >5 mm induration to usual-strength spherulin and were considered skin test-positive. Mononuclear cells from these individuals were more responsive by lymphocyte transformation (12, 541 ± 3746 vs. -112 ± 260 cpm, P =.007) and produced significantly more interleukin-2 (3481 ± 1067 vs.-5±69cpm, P <.001)andinterferon-γ(1831 ±481 vs. 75 ± 58 pg/ml, P <.001) than cells from skin test-negative donors in response to a coccidioi-dal antigen. However, the correlation between the skin test size and the magnitude of the in vitro response among skin test-positive donors was poor (R²= 0.08, P =.24). These results indicate that healthy individuals with dermal hypersensitivity to Coccidioides immitis can be distinguished from those without hypersensitivity by their cellular in vitro response to a coccidioidal antigen.

Original language	English (US)
Pages (from-to)	710-715
Number of pages	6
Journal	Journal of Infectious Diseases
Volume	165
Issue number	4
DOIs	https://doi.org/10.1093/infdis/165.4.710
State	Published - Apr 1 1992

ASJC Scopus subject areas

General Medicine

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Ampel, N. M., Bejarano, G. C., Salas, S. D., & Galgiani, J. N. (1992). In Vitro Assessment of Cellular Immunity in Human Coccidioidomycosis: Relationship between Dermal Hypersensitivity, Lymphocyte Transformation, and Lymphokine Production by Peripheral Blood Mononuclear Cells from Healthy Adults. Journal of Infectious Diseases, 165(4), 710-715. https://doi.org/10.1093/infdis/165.4.710

In Vitro Assessment of Cellular Immunity in Human Coccidioidomycosis: Relationship between Dermal Hypersensitivity, Lymphocyte Transformation, and Lymphokine Production by Peripheral Blood Mononuclear Cells from Healthy Adults. / Ampel, Neil M.; Bejarano, George C.; Salas, Steven D. et al.
In: Journal of Infectious Diseases, Vol. 165, No. 4, 01.04.1992, p. 710-715.

Research output: Contribution to journal › Article › peer-review

Ampel, NM, Bejarano, GC, Salas, SD & Galgiani, JN 1992, 'In Vitro Assessment of Cellular Immunity in Human Coccidioidomycosis: Relationship between Dermal Hypersensitivity, Lymphocyte Transformation, and Lymphokine Production by Peripheral Blood Mononuclear Cells from Healthy Adults', Journal of Infectious Diseases, vol. 165, no. 4, pp. 710-715. https://doi.org/10.1093/infdis/165.4.710

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title = "In Vitro Assessment of Cellular Immunity in Human Coccidioidomycosis: Relationship between Dermal Hypersensitivity, Lymphocyte Transformation, and Lymphokine Production by Peripheral Blood Mononuclear Cells from Healthy Adults",

abstract = "Dermal hypersensitivity in coccidioidomycosis was compared with three simultaneous measures of in vitro cellular immunity using 35 healthy donors living in an area endemic for coccidioidomycosis. Twenty donors had >5 mm induration to usual-strength spherulin and were considered skin test-positive. Mononuclear cells from these individuals were more responsive by lymphocyte transformation (12, 541 ± 3746 vs. -112 ± 260 cpm, P =.007) and produced significantly more interleukin-2 (3481 ± 1067 vs.-5±69cpm, P <.001)andinterferon-γ(1831 ±481 vs. 75 ± 58 pg/ml, P <.001) than cells from skin test-negative donors in response to a coccidioi-dal antigen. However, the correlation between the skin test size and the magnitude of the in vitro response among skin test-positive donors was poor (R2= 0.08, P =.24). These results indicate that healthy individuals with dermal hypersensitivity to Coccidioides immitis can be distinguished from those without hypersensitivity by their cellular in vitro response to a coccidioidal antigen.",

author = "Ampel, {Neil M.} and Bejarano, {George C.} and Salas, {Steven D.} and Galgiani, {John N.}",

note = "Funding Information: Received 14 August 1991; revised 13 November 1991. Presented in part: 30th Interscience Conference on Antimicrobial Agents and Chemotherapy, Atlanta. October 1990 (abstract 322). The study was conducted with the approval ofthe Human Subjects Committee ofthe University ofArizona. Informed consent was obtained from all participants. Grant support: Arizona Lung Association, Department of Veterans Affairs. and Arizona Disease Control Research Commission (82-0689). Reprints or correspondence: Dr. Neil M. Arnpel, Medical Service (III), VAMC, 3601 S. Sixth Ave., Tucson, AZ 85723.",

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N1 - Funding Information: Received 14 August 1991; revised 13 November 1991. Presented in part: 30th Interscience Conference on Antimicrobial Agents and Chemotherapy, Atlanta. October 1990 (abstract 322). The study was conducted with the approval ofthe Human Subjects Committee ofthe University ofArizona. Informed consent was obtained from all participants. Grant support: Arizona Lung Association, Department of Veterans Affairs. and Arizona Disease Control Research Commission (82-0689). Reprints or correspondence: Dr. Neil M. Arnpel, Medical Service (III), VAMC, 3601 S. Sixth Ave., Tucson, AZ 85723.

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N2 - Dermal hypersensitivity in coccidioidomycosis was compared with three simultaneous measures of in vitro cellular immunity using 35 healthy donors living in an area endemic for coccidioidomycosis. Twenty donors had >5 mm induration to usual-strength spherulin and were considered skin test-positive. Mononuclear cells from these individuals were more responsive by lymphocyte transformation (12, 541 ± 3746 vs. -112 ± 260 cpm, P =.007) and produced significantly more interleukin-2 (3481 ± 1067 vs.-5±69cpm, P <.001)andinterferon-γ(1831 ±481 vs. 75 ± 58 pg/ml, P <.001) than cells from skin test-negative donors in response to a coccidioi-dal antigen. However, the correlation between the skin test size and the magnitude of the in vitro response among skin test-positive donors was poor (R2= 0.08, P =.24). These results indicate that healthy individuals with dermal hypersensitivity to Coccidioides immitis can be distinguished from those without hypersensitivity by their cellular in vitro response to a coccidioidal antigen.

AB - Dermal hypersensitivity in coccidioidomycosis was compared with three simultaneous measures of in vitro cellular immunity using 35 healthy donors living in an area endemic for coccidioidomycosis. Twenty donors had >5 mm induration to usual-strength spherulin and were considered skin test-positive. Mononuclear cells from these individuals were more responsive by lymphocyte transformation (12, 541 ± 3746 vs. -112 ± 260 cpm, P =.007) and produced significantly more interleukin-2 (3481 ± 1067 vs.-5±69cpm, P <.001)andinterferon-γ(1831 ±481 vs. 75 ± 58 pg/ml, P <.001) than cells from skin test-negative donors in response to a coccidioi-dal antigen. However, the correlation between the skin test size and the magnitude of the in vitro response among skin test-positive donors was poor (R2= 0.08, P =.24). These results indicate that healthy individuals with dermal hypersensitivity to Coccidioides immitis can be distinguished from those without hypersensitivity by their cellular in vitro response to a coccidioidal antigen.

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In Vitro Assessment of Cellular Immunity in Human Coccidioidomycosis: Relationship between Dermal Hypersensitivity, Lymphocyte Transformation, and Lymphokine Production by Peripheral Blood Mononuclear Cells from Healthy Adults

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