In situ detection of Australian gill-associated virus with a yellow head virus gene probe

Kathy F.J. Tang; Kirsten M. Spann; Leigh Owens; Donald V. Lightner

doi:10.1016/S0044-8486(01)00666-4

In situ detection of Australian gill-associated virus with a yellow head virus gene probe

Kathy F.J. Tang, Kirsten M. Spann, Leigh Owens, Donald V. Lightner

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

A digoxigenin-labeled gene probe for yellow head virus (YHV) was used to detect gill-associated virus (GAV) in Penaeus monodon from Australia via in situ hybridization. In GAV-infected shrimp, positively reacting tissues included: lymphoid organ, gills, antennal gland, and cuticular epithelium of the stomach. This demonstrates that a YHV probe can be used as a diagnostic tool for GAV and supports previous suggestions that these two viruses are closely related.

Original language	English (US)
Pages (from-to)	1-5
Number of pages	5
Journal	Aquaculture
Volume	205
Issue number	1-2
DOIs	https://doi.org/10.1016/S0044-8486(01)00666-4
State	Published - Feb 28 2002

Keywords

Gill-associated virus
In situ hybridization
Yellow head virus

ASJC Scopus subject areas

Aquatic Science

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10.1016/S0044-8486(01)00666-4

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title = "In situ detection of Australian gill-associated virus with a yellow head virus gene probe",

abstract = "A digoxigenin-labeled gene probe for yellow head virus (YHV) was used to detect gill-associated virus (GAV) in Penaeus monodon from Australia via in situ hybridization. In GAV-infected shrimp, positively reacting tissues included: lymphoid organ, gills, antennal gland, and cuticular epithelium of the stomach. This demonstrates that a YHV probe can be used as a diagnostic tool for GAV and supports previous suggestions that these two viruses are closely related.",

keywords = "Gill-associated virus, In situ hybridization, Yellow head virus",

author = "Tang, {Kathy F.J.} and Spann, {Kirsten M.} and Leigh Owens and Lightner, {Donald V.}",

note = "Funding Information: This work was supported by the Gulf Coast Research Laboratory Consortium Marine Shrimp Farming Program, CSRS, USDA under Grant No. 95-38808-1424.",

year = "2002",

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T1 - In situ detection of Australian gill-associated virus with a yellow head virus gene probe

AU - Tang, Kathy F.J.

AU - Spann, Kirsten M.

AU - Owens, Leigh

AU - Lightner, Donald V.

N1 - Funding Information: This work was supported by the Gulf Coast Research Laboratory Consortium Marine Shrimp Farming Program, CSRS, USDA under Grant No. 95-38808-1424.

PY - 2002/2/28

Y1 - 2002/2/28

N2 - A digoxigenin-labeled gene probe for yellow head virus (YHV) was used to detect gill-associated virus (GAV) in Penaeus monodon from Australia via in situ hybridization. In GAV-infected shrimp, positively reacting tissues included: lymphoid organ, gills, antennal gland, and cuticular epithelium of the stomach. This demonstrates that a YHV probe can be used as a diagnostic tool for GAV and supports previous suggestions that these two viruses are closely related.

AB - A digoxigenin-labeled gene probe for yellow head virus (YHV) was used to detect gill-associated virus (GAV) in Penaeus monodon from Australia via in situ hybridization. In GAV-infected shrimp, positively reacting tissues included: lymphoid organ, gills, antennal gland, and cuticular epithelium of the stomach. This demonstrates that a YHV probe can be used as a diagnostic tool for GAV and supports previous suggestions that these two viruses are closely related.

KW - Gill-associated virus

KW - In situ hybridization

KW - Yellow head virus

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In situ detection of Australian gill-associated virus with a yellow head virus gene probe

Abstract

Keywords

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