In-cell RT-PCR in a single, detached plant cell

Ho Hyung Woo; Lindy A. Brigham; Martha C. Hawes

doi:10.1007/BF02669191

In-cell RT-PCR in a single, detached plant cell

Ho Hyung Woo, Lindy A. Brigham, Martha C. Hawes

Environmental Science, Research

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

A procedure is presented for the detection of mRNA in a single root border cell of pea seedlings using RT-PCR (reverse transcription-polymerase chain reaction) and direct incorporation of digoxigenin-dUTP. It includes cell preservation with aldehyde. Signal is detected using a conjugate of anti-DIG and alkaline phosphatase.

Original language	English (US)
Pages (from-to)	355-362
Number of pages	8
Journal	Plant Molecular Biology Reporter
Volume	13
Issue number	4
DOIs	https://doi.org/10.1007/BF02669191
State	Published - Dec 1995

Keywords

In-cell RT-PCR
digoxigenin-dUTP
histone H1
single detached plant cell

ASJC Scopus subject areas

Molecular Biology
Plant Science

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10.1007/BF02669191

Cite this

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title = "In-cell RT-PCR in a single, detached plant cell",

abstract = "A procedure is presented for the detection of mRNA in a single root border cell of pea seedlings using RT-PCR (reverse transcription-polymerase chain reaction) and direct incorporation of digoxigenin-dUTP. It includes cell preservation with aldehyde. Signal is detected using a conjugate of anti-DIG and alkaline phosphatase.",

keywords = "In-cell RT-PCR, digoxigenin-dUTP, histone H1, single detached plant cell",

author = "Woo, {Ho Hyung} and Brigham, {Lindy A.} and Hawes, {Martha C.}",

year = "1995",

month = dec,

doi = "10.1007/BF02669191",

language = "English (US)",

volume = "13",

pages = "355--362",

journal = "Plant Molecular Biology Reporter",

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TY - JOUR

T1 - In-cell RT-PCR in a single, detached plant cell

AU - Woo, Ho Hyung

AU - Brigham, Lindy A.

AU - Hawes, Martha C.

PY - 1995/12

Y1 - 1995/12

N2 - A procedure is presented for the detection of mRNA in a single root border cell of pea seedlings using RT-PCR (reverse transcription-polymerase chain reaction) and direct incorporation of digoxigenin-dUTP. It includes cell preservation with aldehyde. Signal is detected using a conjugate of anti-DIG and alkaline phosphatase.

AB - A procedure is presented for the detection of mRNA in a single root border cell of pea seedlings using RT-PCR (reverse transcription-polymerase chain reaction) and direct incorporation of digoxigenin-dUTP. It includes cell preservation with aldehyde. Signal is detected using a conjugate of anti-DIG and alkaline phosphatase.

KW - In-cell RT-PCR

KW - digoxigenin-dUTP

KW - histone H1

KW - single detached plant cell

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U2 - 10.1007/BF02669191

DO - 10.1007/BF02669191

M3 - Article

AN - SCOPUS:51249168781

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EP - 362

JO - Plant Molecular Biology Reporter

JF - Plant Molecular Biology Reporter

IS - 4

ER -

In-cell RT-PCR in a single, detached plant cell

Abstract

Keywords

ASJC Scopus subject areas

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