Improvement of PCR method for the detection of monodon baculovirus (MBV) in penaeid shrimp

Win Surachetpong; Bonnie T. Poulos; Kathy F.J. Tang; Donald V. Lightner

doi:10.1016/j.aquaculture.2005.04.051

Improvement of PCR method for the detection of monodon baculovirus (MBV) in penaeid shrimp

Win Surachetpong, Bonnie T. Poulos, Kathy F.J. Tang, Donald V. Lightner

Research output: Contribution to journal › Article › peer-review

25 Scopus citations

Abstract

Penaeus monodon-type baculovirus (MBV) infects and causes disease in hatchery-reared larvae and early stages of juvenile P. monodon. In this study, an improved 1-step PCR method was developed for the detection of MBV. The oligonucleotide primers, assigned as 261F and 261R, were designed from a genomic clone of MBV. Using extracted DNA from hepatopancreas or feces of ten geographic isolates of MBV infected shrimp, the PCR reaction yields a 261 bp fragment specific to the MBV sequence. Primers 261F/R did not cross-react with DNA from other shrimp viruses or with uninfected shrimp tissue. The detection limit of this primer pair is 100 copies of positive control plasmid DNA. This improved molecular method provides a diagnostic tool for identification of MBV in infected shrimp tissue collected from different geographic regions.

Original language	English (US)
Pages (from-to)	69-75
Number of pages	7
Journal	Aquaculture
Volume	249
Issue number	1-4
DOIs	https://doi.org/10.1016/j.aquaculture.2005.04.051
State	Published - Sep 12 2005

Keywords

Monodon baculovirus (MBV)
PCR
Penaeid shrimp

ASJC Scopus subject areas

Aquatic Science

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10.1016/j.aquaculture.2005.04.051

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@article{84d0ecc0568a4b278b21aaedc8850241,

title = "Improvement of PCR method for the detection of monodon baculovirus (MBV) in penaeid shrimp",

abstract = "Penaeus monodon-type baculovirus (MBV) infects and causes disease in hatchery-reared larvae and early stages of juvenile P. monodon. In this study, an improved 1-step PCR method was developed for the detection of MBV. The oligonucleotide primers, assigned as 261F and 261R, were designed from a genomic clone of MBV. Using extracted DNA from hepatopancreas or feces of ten geographic isolates of MBV infected shrimp, the PCR reaction yields a 261 bp fragment specific to the MBV sequence. Primers 261F/R did not cross-react with DNA from other shrimp viruses or with uninfected shrimp tissue. The detection limit of this primer pair is 100 copies of positive control plasmid DNA. This improved molecular method provides a diagnostic tool for identification of MBV in infected shrimp tissue collected from different geographic regions.",

keywords = "Monodon baculovirus (MBV), PCR, Penaeid shrimp",

author = "Win Surachetpong and Poulos, {Bonnie T.} and Tang, {Kathy F.J.} and Lightner, {Donald V.}",

note = "Funding Information: This work was supported by Gulf Coast Research Laboratory Consortium Marine Shrimp Farming Program, CSREES, USDA under grant no. 2002-38808-01345.",

year = "2005",

month = sep,

day = "12",

doi = "10.1016/j.aquaculture.2005.04.051",

language = "English (US)",

volume = "249",

pages = "69--75",

journal = "Aquaculture",

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AU - Surachetpong, Win

AU - Poulos, Bonnie T.

AU - Tang, Kathy F.J.

AU - Lightner, Donald V.

N1 - Funding Information: This work was supported by Gulf Coast Research Laboratory Consortium Marine Shrimp Farming Program, CSREES, USDA under grant no. 2002-38808-01345.

PY - 2005/9/12

Y1 - 2005/9/12

N2 - Penaeus monodon-type baculovirus (MBV) infects and causes disease in hatchery-reared larvae and early stages of juvenile P. monodon. In this study, an improved 1-step PCR method was developed for the detection of MBV. The oligonucleotide primers, assigned as 261F and 261R, were designed from a genomic clone of MBV. Using extracted DNA from hepatopancreas or feces of ten geographic isolates of MBV infected shrimp, the PCR reaction yields a 261 bp fragment specific to the MBV sequence. Primers 261F/R did not cross-react with DNA from other shrimp viruses or with uninfected shrimp tissue. The detection limit of this primer pair is 100 copies of positive control plasmid DNA. This improved molecular method provides a diagnostic tool for identification of MBV in infected shrimp tissue collected from different geographic regions.

AB - Penaeus monodon-type baculovirus (MBV) infects and causes disease in hatchery-reared larvae and early stages of juvenile P. monodon. In this study, an improved 1-step PCR method was developed for the detection of MBV. The oligonucleotide primers, assigned as 261F and 261R, were designed from a genomic clone of MBV. Using extracted DNA from hepatopancreas or feces of ten geographic isolates of MBV infected shrimp, the PCR reaction yields a 261 bp fragment specific to the MBV sequence. Primers 261F/R did not cross-react with DNA from other shrimp viruses or with uninfected shrimp tissue. The detection limit of this primer pair is 100 copies of positive control plasmid DNA. This improved molecular method provides a diagnostic tool for identification of MBV in infected shrimp tissue collected from different geographic regions.

KW - Monodon baculovirus (MBV)

KW - PCR

KW - Penaeid shrimp

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JF - Aquaculture

IS - 1-4

ER -

Improvement of PCR method for the detection of monodon baculovirus (MBV) in penaeid shrimp

Abstract

Keywords

ASJC Scopus subject areas

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