Improvement of a PCR method for the detection of necrotizing hepatopancreatitis in shrimp

Linda M. Nunan, Carlos Pantoja, Donald V. Lightner

    Research output: Contribution to journalArticlepeer-review

    17 Scopus citations

    Abstract

    Necrotizing hepatopancreatitis (NHP) is considered to be one of the most important bacterial diseases affecting penaeid shrimp culture and is caused by an unclassified Gram-negative, pleomorphic, intracellular Alphaproteobacterium. Due to the enteric nature of the bacteria, PCR is the one non-lethal method available for detection of the pathogen. Over a decade ago, a PCR protocol was developed for detection of NHP, which over the subsequent years was shown to occasionally generate false positive reactions. The University of Arizona Aquaculture Pathology Laboratory has developed a set of primers and PCR cycling parameters that have been tested on a variety of DNA templates, using 2 types of PCR reagent systems, which eliminated the generation of false positive amplicons.

    Original languageEnglish (US)
    Pages (from-to)69-73
    Number of pages5
    JournalDiseases of aquatic organisms
    Volume80
    Issue number1
    DOIs
    StatePublished - Jun 19 2008

    Keywords

    • NHP
    • Necrotizing hepatopancreatitis
    • PCR
    • Penaeus vannamei
    • Shrimp

    ASJC Scopus subject areas

    • Ecology, Evolution, Behavior and Systematics
    • Aquatic Science

    Fingerprint

    Dive into the research topics of 'Improvement of a PCR method for the detection of necrotizing hepatopancreatitis in shrimp'. Together they form a unique fingerprint.

    Cite this