Improved protection of mice against lethal respiratory infection with Coccidioides posadasii using two recombinant antigens expressed as a single protein

Lisa F. Shubitz; Jieh Juen Yu; Chiung Yu Hung; Theo N. Kirkland; Tao Peng; Robert Perrill; Julie Simons; Jianmin Xue; Roger A. Herr; Garry T. Cole; John N. Galgiani

doi:10.1016/j.vaccine.2006.04.002

Improved protection of mice against lethal respiratory infection with Coccidioides posadasii using two recombinant antigens expressed as a single protein

Lisa F. Shubitz
, Jieh Juen Yu
, Chiung Yu Hung
, Theo N. Kirkland
, Tao Peng
, Robert Perrill
, Julie Simons
, Jianmin Xue
, Roger A. Herr
, Garry T. Cole
, John N. Galgiani

Research output: Contribution to journal › Article › peer-review

58 Scopus citations

Abstract

Two recombinant antigens which individually protect mice from lethal intranasal infection were studied in combination, either as a mixture of two separately expressed proteins or as a single chimeric expression product. Mice vaccinated with either combination survived longer than mice given single antigens. Immunized mice also exhibited specific IgG immunoglobulins and yielded splenocytes which produced interferon-γ in response to either antigen. The chimeric antigen has the practical advantage of offering enhanced protection from multiple components without increasing production costs.

Original language	English (US)
Pages (from-to)	5904-5911
Number of pages	8
Journal	Vaccine
Volume	24
Issue number	31-32
DOIs	https://doi.org/10.1016/j.vaccine.2006.04.002
State	Published - Jul 26 2006

Keywords

Cell-mediated immunity
Coccidioides
Mouse
Recombinant expression protein
Vaccine

ASJC Scopus subject areas

Molecular Medicine
General Immunology and Microbiology
General Veterinary
Public Health, Environmental and Occupational Health
Infectious Diseases

Access to Document

10.1016/j.vaccine.2006.04.002

Cite this

Shubitz, L. F., Yu, J. J., Hung, C. Y., Kirkland, T. N., Peng, T., Perrill, R., Simons, J., Xue, J., Herr, R. A., Cole, G. T., & Galgiani, J. N. (2006). Improved protection of mice against lethal respiratory infection with Coccidioides posadasii using two recombinant antigens expressed as a single protein. Vaccine, 24(31-32), 5904-5911. https://doi.org/10.1016/j.vaccine.2006.04.002

Shubitz, LF, Yu, JJ, Hung, CY, Kirkland, TN, Peng, T, Perrill, R, Simons, J, Xue, J, Herr, RA, Cole, GT & Galgiani, JN 2006, 'Improved protection of mice against lethal respiratory infection with Coccidioides posadasii using two recombinant antigens expressed as a single protein', Vaccine, vol. 24, no. 31-32, pp. 5904-5911. https://doi.org/10.1016/j.vaccine.2006.04.002

@article{03448f0cb75747668814b862ad954441,

title = "Improved protection of mice against lethal respiratory infection with Coccidioides posadasii using two recombinant antigens expressed as a single protein",

abstract = "Two recombinant antigens which individually protect mice from lethal intranasal infection were studied in combination, either as a mixture of two separately expressed proteins or as a single chimeric expression product. Mice vaccinated with either combination survived longer than mice given single antigens. Immunized mice also exhibited specific IgG immunoglobulins and yielded splenocytes which produced interferon-γ in response to either antigen. The chimeric antigen has the practical advantage of offering enhanced protection from multiple components without increasing production costs.",

keywords = "Cell-mediated immunity, Coccidioides, Mouse, Recombinant expression protein, Vaccine",

author = "Shubitz, \{Lisa F.\} and Yu, \{Jieh Juen\} and Hung, \{Chiung Yu\} and Kirkland, \{Theo N.\} and Tao Peng and Robert Perrill and Julie Simons and Jianmin Xue and Herr, \{Roger A.\} and Cole, \{Garry T.\} and Galgiani, \{John N.\}",

note = "Funding Information: This work was supported in part by the US Department of Veterans Affairs, the California Health Care Foundation, and PHS Research Grants 5PO1AI37232-06 and 1PO1AI061310-01 from the National Institutes of Health. ",

year = "2006",

month = jul,

day = "26",

doi = "10.1016/j.vaccine.2006.04.002",

language = "English (US)",

volume = "24",

pages = "5904--5911",

journal = "Vaccine",

issn = "0264-410X",

publisher = "Elsevier Ltd",

number = "31-32",

}

TY - JOUR

T1 - Improved protection of mice against lethal respiratory infection with Coccidioides posadasii using two recombinant antigens expressed as a single protein

AU - Shubitz, Lisa F.

AU - Yu, Jieh Juen

AU - Hung, Chiung Yu

AU - Kirkland, Theo N.

AU - Peng, Tao

AU - Perrill, Robert

AU - Simons, Julie

AU - Xue, Jianmin

AU - Herr, Roger A.

AU - Cole, Garry T.

AU - Galgiani, John N.

N1 - Funding Information: This work was supported in part by the US Department of Veterans Affairs, the California Health Care Foundation, and PHS Research Grants 5PO1AI37232-06 and 1PO1AI061310-01 from the National Institutes of Health.

PY - 2006/7/26

Y1 - 2006/7/26

N2 - Two recombinant antigens which individually protect mice from lethal intranasal infection were studied in combination, either as a mixture of two separately expressed proteins or as a single chimeric expression product. Mice vaccinated with either combination survived longer than mice given single antigens. Immunized mice also exhibited specific IgG immunoglobulins and yielded splenocytes which produced interferon-γ in response to either antigen. The chimeric antigen has the practical advantage of offering enhanced protection from multiple components without increasing production costs.

AB - Two recombinant antigens which individually protect mice from lethal intranasal infection were studied in combination, either as a mixture of two separately expressed proteins or as a single chimeric expression product. Mice vaccinated with either combination survived longer than mice given single antigens. Immunized mice also exhibited specific IgG immunoglobulins and yielded splenocytes which produced interferon-γ in response to either antigen. The chimeric antigen has the practical advantage of offering enhanced protection from multiple components without increasing production costs.

KW - Cell-mediated immunity

KW - Coccidioides

KW - Mouse

KW - Recombinant expression protein

KW - Vaccine

UR - https://www.scopus.com/pages/publications/33745364561

UR - https://www.scopus.com/pages/publications/33745364561#tab=citedBy

U2 - 10.1016/j.vaccine.2006.04.002

DO - 10.1016/j.vaccine.2006.04.002

M3 - Article

C2 - 16759762

AN - SCOPUS:33745364561

SN - 0264-410X

VL - 24

SP - 5904

EP - 5911

JO - Vaccine

JF - Vaccine

IS - 31-32

ER -

Improved protection of mice against lethal respiratory infection with Coccidioides posadasii using two recombinant antigens expressed as a single protein

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this