Improved protection of mice against lethal respiratory infection with Coccidioides posadasii using two recombinant antigens expressed as a single protein

Lisa F. Shubitz; Jieh Juen Yu; Chiung Yu Hung; Theo N. Kirkland; Tao Peng; Robert Perrill; Julie Simons; Jianmin Xue; Roger A. Herr; Garry T. Cole; John N. Galgiani

doi:10.1016/j.vaccine.2006.04.002

Improved protection of mice against lethal respiratory infection with Coccidioides posadasii using two recombinant antigens expressed as a single protein

Lisa F. Shubitz, Jieh Juen Yu, Chiung Yu Hung, Theo N. Kirkland, Tao Peng, Robert Perrill, Julie Simons, Jianmin Xue, Roger A. Herr, Garry T. Cole, John N. Galgiani

Research output: Contribution to journal › Article › peer-review

55 Scopus citations

Abstract

Two recombinant antigens which individually protect mice from lethal intranasal infection were studied in combination, either as a mixture of two separately expressed proteins or as a single chimeric expression product. Mice vaccinated with either combination survived longer than mice given single antigens. Immunized mice also exhibited specific IgG immunoglobulins and yielded splenocytes which produced interferon-γ in response to either antigen. The chimeric antigen has the practical advantage of offering enhanced protection from multiple components without increasing production costs.

Original language	English (US)
Pages (from-to)	5904-5911
Number of pages	8
Journal	Vaccine
Volume	24
Issue number	31-32
DOIs	https://doi.org/10.1016/j.vaccine.2006.04.002
State	Published - Jul 26 2006

Keywords

Cell-mediated immunity
Coccidioides
Mouse
Recombinant expression protein
Vaccine

ASJC Scopus subject areas

Molecular Medicine
General Immunology and Microbiology
General Veterinary
Public Health, Environmental and Occupational Health
Infectious Diseases

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10.1016/j.vaccine.2006.04.002

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Shubitz, L. F., Yu, J. J., Hung, C. Y., Kirkland, T. N., Peng, T., Perrill, R., Simons, J., Xue, J., Herr, R. A., Cole, G. T., & Galgiani, J. N. (2006). Improved protection of mice against lethal respiratory infection with Coccidioides posadasii using two recombinant antigens expressed as a single protein. Vaccine, 24(31-32), 5904-5911. https://doi.org/10.1016/j.vaccine.2006.04.002

Shubitz, LF, Yu, JJ, Hung, CY, Kirkland, TN, Peng, T, Perrill, R, Simons, J, Xue, J, Herr, RA, Cole, GT & Galgiani, JN 2006, 'Improved protection of mice against lethal respiratory infection with Coccidioides posadasii using two recombinant antigens expressed as a single protein', Vaccine, vol. 24, no. 31-32, pp. 5904-5911. https://doi.org/10.1016/j.vaccine.2006.04.002

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abstract = "Two recombinant antigens which individually protect mice from lethal intranasal infection were studied in combination, either as a mixture of two separately expressed proteins or as a single chimeric expression product. Mice vaccinated with either combination survived longer than mice given single antigens. Immunized mice also exhibited specific IgG immunoglobulins and yielded splenocytes which produced interferon-γ in response to either antigen. The chimeric antigen has the practical advantage of offering enhanced protection from multiple components without increasing production costs.",

keywords = "Cell-mediated immunity, Coccidioides, Mouse, Recombinant expression protein, Vaccine",

author = "Shubitz, {Lisa F.} and Yu, {Jieh Juen} and Hung, {Chiung Yu} and Kirkland, {Theo N.} and Tao Peng and Robert Perrill and Julie Simons and Jianmin Xue and Herr, {Roger A.} and Cole, {Garry T.} and Galgiani, {John N.}",

note = "Funding Information: This work was supported in part by the US Department of Veterans Affairs, the California Health Care Foundation, and PHS Research Grants 5PO1AI37232-06 and 1PO1AI061310-01 from the National Institutes of Health. ",

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doi = "10.1016/j.vaccine.2006.04.002",

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AU - Yu, Jieh Juen

AU - Hung, Chiung Yu

AU - Kirkland, Theo N.

AU - Peng, Tao

AU - Perrill, Robert

AU - Simons, Julie

AU - Xue, Jianmin

AU - Herr, Roger A.

AU - Cole, Garry T.

AU - Galgiani, John N.

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KW - Recombinant expression protein

KW - Vaccine

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Improved protection of mice against lethal respiratory infection with Coccidioides posadasii using two recombinant antigens expressed as a single protein

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Keywords

ASJC Scopus subject areas

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