Improved method of porcine pancreas procurement with arterial flush and ductal injection enhances islet isolation outcome

T. Anazawa, A. N. Balamurugan, K. K. Papas, E. S. Avgoustiniatos, J. Ferrer, S. Matsumoto, D. E.R. Sutherland, B. J. Hering

Research output: Contribution to journalArticlepeer-review

15 Scopus citations


Background: Several pancreas procurement procedures have been used for porcine islet isolation; however, their impact on outcomes has not been extensively studied. We evaluated an advanced procurement technique for porcine islet isolation designed to reduce warm ischemia, to remove blood content, and enhance cooling of the pancreas by implementing a vascular flush and ductal preservation. Method: Pancreata procured from adult Landrace pigs were divided into 3 different surgical protocols: Pancreatectomy utilizing only surface cooling (group 1; n = 24); surface cooling and ductal injection with cold preservation solution before pancreatectomy (group 2; n = 12); or surface cooling, ductal injection, and an approach by selectively flushing through the celiac trunk and the superior mesenteric artery (group 3; n = 14). We assessed the islet isolation results and quality using in vitro and in vivo assays. Results: Significantly higher overall yield and islet yield per gram pancreas were obtained from group 3 pigs compared with the other groups. Measurements of islet viability after 7 days of culture, as assessed by oxygen consumption rate per DNA, showed that group 3 islets displayed the highest values. Sustained normoglycemia was observed in diabetic nude mice transplanted with 2000 islet equivalents from all 3 groups. Discussion: This study demonstrated that an advanced pancreas procurement technique including ductal preservation and selective arterial flush with cold preservation solution provided significant improvements in porcine islet isolation outcomes.

Original languageEnglish (US)
Pages (from-to)2032-2035
Number of pages4
JournalTransplantation Proceedings
Issue number6
StatePublished - Jul 2010

ASJC Scopus subject areas

  • Surgery
  • Transplantation


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