Improved biochemical preservation of lung slices during cold storage

David A. Bull, Rafe C. Connors, Bruce B. Reid, Aida Albanil, James C. Stringham, Shreekanth V. Karwande

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Background. Development of lung preservation solutions typically requires whole-organ models which are animal and labor intensive. These models rely on physiologic rather than biochemical endpoints, making accurate comparison of the relative efficacy of individual solution components difficult. We hypothesized that lung slices could be used to assess preservation of biochemical function during cold storage. Materials and methods. Whole rat lungs were precision cut into slices with a thickness of 500 μm and preserved at 4°C in the following solutions: University of Wisconsin (UW), Euro-Collins (EC), low-potassium-dextran (LPD), Kyoto (K), normal saline (NS), or a novel lung preservation solution (NPS) developed using this model. Lung biochemical function was assessed by ATP content (ηmol ATP/mg wet wt) and capacity for protein synthesis (cpm/mg protein) immediately following slicing (0 h) and at 6, 12, 18, and 24 h of cold storage. Six slices were assayed at each time point for each solution. The data were analyzed using analysis of variance and are presented as means ± SD. Results. ATP content was significantly higher in the lung slices stored in NPS compared with all other solutions at each time point (P < 0.0001). Protein synthesis was significantly higher in the lung slices stored in NPS compared with all other solutions at 6, 12, and 18 h of preservation (P < 0.05). Conclusions. This lung slice model allows the rapid and efficient screening of lung preservation solutions and their components using quantifiable biochemical endpoints. Using this model, we have developed a novel solution that improves the biochemical preservation of lung slices during cold storage. 2000 Academic Press.

Original languageEnglish (US)
Pages (from-to)144-148
Number of pages5
JournalJournal of Surgical Research
Volume90
Issue number2
DOIs
StatePublished - May 15 2000
Externally publishedYes

Keywords

  • ATP
  • Lung preservation
  • Lung preservation solutions
  • Lung tissue slices
  • Protein synthesis

ASJC Scopus subject areas

  • Surgery

Fingerprint

Dive into the research topics of 'Improved biochemical preservation of lung slices during cold storage'. Together they form a unique fingerprint.

Cite this